Hamamatsu University School of Medicine

Nikon Corporation

Yokogawa Electric Corporation

Takashi Sakurai

Susumu Terakawa

Takeo Tanaami

Yasujiro Kiyota

Hisao Osawa

Hideo Hirukawa

Hideo Mogami

Yoshihiko Wakazono

Remi Susuki

The observing apparatus is being equipped with an image-forming optical system which forms an image of light emitted from a specimen, an imaging unit which picks up the image of the specimen formed by the image-forming optical system, and an illuminating unit which illuminates the specimen with a surface illuminant in which bright areas and dark areas are arranged alternately in order to provide an observing apparatus suitable to observe a transparent specimen with a wide field of view. If the position of the surface illuminant and the pitch of contrasting are set properly, each partial area of the specimen is illuminated obliquely at a small angle by each bright area of the surface illuminant. Therefore, the imaging unit can acquire a dark-field observation image of each partial area.

Observing device

To provide a cell sorting method and a cell sorter which are capable of performing sorting focusing on various changes in each cell as stress on living cells is kept as low as possible, and proliferating the sorted cells. The present invention performs cell sorting using a fiber unit in which a plurality of fibers is bundled so as to be separable and unitable. In the present invention, a cell group containing target cells targeted for sorting is dispersed in a fluid into which the fiber unit is immersed, target fibers to which the target cells are attached among the plurality of fibers, are separated from the fiber unit, and the target cells are proliferated by use of the separated target fibers.

Cell sorting method and cell sorter

A switch-type imaging fiber apparatus and a branch-type imaging fiber apparatus are provided. Light emitted from a laser light source is made incident on an opening of an IF (image fiber) guided by a GM (galvanometer), output from an optical system provided at a leading end portion of the IF, and applied to an observation target. The light reflected by the observation target is sent back to the IF, transformed into parallel light by an f&#x3b8; objective lens, reflected by a dichroic mirror, made incident on an image pickup device (CCD) via an imaging lens, and can be observed on a monitor. The GM is controlled to switch to make the light incident on the opening of an arbitrary one of a plurality of IFs. Accordingly, observation can be performed by focusing the light solely on the arbitrary IF.

Switch-type imaging fiber apparatus and branch-type imaging fiber apparatus

Growing community of inventors

Hamamatsu, Japan

Takashi Sakurai