Hoya Corporation

Lsi Medience Corporation

Hoya Magnetics Singapore Pte. Ltd.

Mediford Corporation

Takashi Morikawa

Teiichiro Umezawa

Hiroshi Tomiyasu

Hiroaki Inoue

Masato Kobayashi

Masafumi Ishiyama

Hideaki Kakinuma

Tsuyoshi Watanabe

Genshichi Hata

Yukiko Shimada

Masashi Suzuki

Satoshi Yokota

A method of screening for a substance that acts on a cell mass includes producing a cell mass by three-dimensional culture of primary cancer cells using a tumor tissue, adding a test substance to the cell mass, and evaluating an action of the test substance on the cell mass. The cell mass is produced by culturing cells obtained from the tumor tissue in a medium containing a 5% v/v or less extracellular matrix on a substantially low-adhesive cell culture substrate and producing the cell mass of the primary cancer cells.

Method of screening for a substance that acts on a cell mass

A method of producing a cell mass by three-dimensional culture of primary cancer cells having proliferative ability and properties of handleability, versatility, and high-throughput performance, in which a tumor tissue is used as a starting material, proliferation of cells such as fibroblasts other than cancer cells is inhibited, and the cell mass includes primary cancer cells as a main component. The object is achieved by providing a method of producing a cell mass by three-dimensional culture of primary cancer cells using a tumor tissue, including: a three-dimensional culture step of culturing cells obtained from the tumor tissue in a medium containing a 5% v/v or less extracellular matrix on a substantially low-adhesive cell culture substrate.

Three-dimensional culture of primary cancer cells using tumor tissue

There is disclosed a magnetic recording medium in which a seed layer, under layer, intermediate layer, first magnetic layer, nonmagnetic layer, second magnetic layer, protective layer, and lubricant layer are successively laminated on a glass substrate, the nonmagnetic layer is constituted of an alloy containing Cr and C, and the magnetic layer is constituted of an alloy containing Co and Pt. The under layer includes at least the seed layer for finely dividing the crystal particles of the magnetic layer, the seed layer includes at least two or more layers of nonmagnetic films, and the intermediate layer formed of the material different from that of the nonmagnetic film is interposed between the nonmagnetic films. In measurement of the thermal stability of the magnetic recording medium, a head is used, the head includes a read/write element, and a write track width is twice or more as large as a read track width in the head.

Magnetic recording medium, and thermal stability measuring method and apparatus of magnetic recording medium

In a magnetic recording medium which comprises a substrate (), a magnetic layer () formed on the substrate, a carbon-based protection layer () comprising carbon and formed on the magnetic layer, and a lubrication layer () formed on the carbon-based protection layer, the carbon-based protection layer comprises a hydrogenated carbon protection layer () facing the magnetic layer and a nitrogenated carbon protection layer () facing the lubrication layer. The hydrogenated carbon protection layer () comprises carbon and hydrogen. The nitrogenated carbon protection layer () includes no hydrogen but comprises carbon and nitrogen.

Magnetic recording medium with multi-portion carbon-based protection layer, and apparatus thereof

Magnetic recording medium

Growing community of inventors

Tokyo, Japan

Takashi Morikawa