Meiji Milk Products Company, Limited

Director of National Institute of Animal Industry, Ministry of

Takashi Kuwana

Atsushi Tajima

Mitsuru Naito

Koichiro Hashimoto

Akira Nakanishi

Yoshiaki Yasuda

A method of culturing avian cells in a medium with a pH value of 7.8 or above, whereby avian cells can be subcultured stably for long. Since avian cells have been incapable of subculture heretofore, it has been impossible to effect in vitro cell or gene manipulation therewith. Now that such a manipulation becomes possible by the invention method, the way is opened for the industrial utilization of avian cells in the creation of transgenic birds and the production of vaccines.

Method of subculturing culturing avian cells at pH 7.8 or above

Cryopreservation of primordial germ cells and germ cells, in which primordial germ cells or germ cells are separated from bird embryos before meiosis and are then suspended and frozen in a freezing medium containing a cryoprotective agent is useful for the conservation of species of birds, for the protection of rare species which are in danger of extinction and also for the production of transgenic animals, the production of novel animals having gene markers on a cellular level, the production of animals useful as disease models, the production of useful substances by genetic engineering, and the development of test methods for toxicity to germ-line cells.

Method for cryopreservation of primordial germ cells and germ cells

Growing community of inventors

Kumamoto, Japan

Takashi Kuwana