Asahi Kasei Pharma Corporation

Ichibiki Co., Ltd.

Takashi Kuroyanagi

Takeshi Matsuoka

Takuji Kouzuma

Atsuhisa Nishimura

Toshihiko Kumazawa

Shinji Koga

Issei Yoshioka

Homare Itou

There is provided a method for specifically determining a glycated &#x3b2;-chain N-terminal of glycated hemoglobin using enzymes without a separation operation, and a determination reagent kit therefor. A protease that cleaves a glycated amino acid and/or a glycated peptide from a glycated &#x3b2;-chain N-terminal without substantially cleaving a glycated amino acid or a glycated peptide from a glycated &#x3b1;-chain N-terminal of glycated hemoglobin or a fragment thereof is screened. The method of specifically determining a glycated &#x3b2;-chain N-terminal of glycated hemoglobin and the determination reagent kit are provided by using the protease obtained by the screening method. According to the present invention, a glycated &#x3b2;-chain N-terminal of glycated hemoglobin can specifically be determined without a separation operation.

Hemoglobin A1c determination method, enzyme to be used therefor, and production method thereof

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Aichi, Japan

Takashi Kuroyanagi