Mitsubishi Kasei Corporation

Takao Uchiyama

Mishio Kawamura

Haruyuki Ohkishi

Makoto Ueda

Reiko Sashida

Sachiko Ohba

Naoshi Imaki

Hiroshi Kusano

Akira Kamata

Yuki Takuma

Kuniji Tanaka

Eri Hosono

Masato Aiura

An agent for capturing metal ions, which consists essentially of a cyclic inulooligosaccharide having from 6 to 8 molecules of fructose bonded by .beta.-2,1 bonds to form a cyclic structure, wherein hydroxyl groups may all or partially be alkylated.

Method for capturing metal ions

The present invention is directed to a method of manufacturing inulotriose and/or inulotetrose by using an exo-type hydrolase capable of hydrolyzing a fructan only every 3 or 4 sugar units from a terminal fructose thereof, so as to produce inulotriose and/or inulotetrose, and of recovering the inulotriose and/or inulotetrose. The enzyme is produced by Streptomyces sp. MCI 2423 (FERM BP-2678) and Aureobacterium sp. MCI 2494 (FERM BP-2679).

Method of manufacturing inulotriose and/or inulotetrose using an

Disclosed herein is an immobilized enzyme inulin-D-fructotransferase carried in a specific anionic exchange resin with pores having a mode radius in the range of 75 to 2,000 angstroms. The immobilized enzyme of the present invention is effective in the production of DFA III from inulin.

Immobilized FTF enzymes

The present invention is directed to an exo-type hydrolase capable of hydrolyzing a fructan only every 3 or 4 sugar units from a terminal fructose thereof to produce predominantly inulotriose and/or inulotetrose. The enzyme is produced by Streptomyces sp. MCI 2423 (FERM BP-2678) and Aureobacterium sp. MCI 2494 (FERM BP-2679).

Exo-type hydrolase capable of hydrolyzing a fructan only every 3 or 4

Disclosed herein is a process for preparing difructose dianhydride III (DFA III) comprising reacting inulin with an inulin lytic enzyme derived from a microorganism belonging to Arthrobacter ilicis. The enzyme employed herein efficiently produces DFA III from inulin and is more stable against heat than conventional enzymes. The present process enables industrial continuous production of DFA III. The preferred strain used herein is Arthrobacter ilicis MCI 2297 (FERM P-9893).

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Minoo, Japan

Takao Uchiyama