Mitsubishi Rayon Company, Limited

Mitsubishi Chemical Corporation

Takanori Akiyama

Tetsuji Nakamura

Eiji Sato

Wataru Mizunashi

Fujio Yu

Yasuhisa Asano

Fumiaki Watanabe

Aya Kitahara

Osamu Katoh

Miki Wakamatsu

Yu Fujio

Yuya Takikawa

Provided is a novel improved nitrile hydratase with improved resistance to amide compounds under high temperatures. Specifically provided is a nitrile hydratase having at least one amino acid mutation selected from (a) to (d) below, in the amino acid sequence expressed in SEQ ID NO:50 (Xto Xrepresent independent arbitrarily-defined amino acid residuals). (a) Xis valine or glycine (b) Xis valine or threonine (c) Xis an amino acid selected from a group consisting of isoleucine, leucine, methionine and threonine, (d) Xis leucine.

Nitrile hydratase

The present invention provides a modified ethylenediamine-N,N'-disuccinate:ethylenediamine lyase. The present invention also provides a protein that comprises the amino acid sequence represented by SEQ ID NO: 1; or a protein that comprises an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO: 1 by deletion, substitution, or addition of one or more amino acid residues, and has an ethylenediamine-N,N&#x2032;-disuccinate:ethylenediamine lyase activity.

Modified ethylenediamine-N,N'-disuccinate:ethylenediamine lyase

Modified ethylenediamine-N, N'-disuccinate: ethylenediamine lyase

The present invention provides an industrially useful improved halohydrin epoxidase, a method for producing the same, and a method for producing an epihalohydrin or 4-halo-3-hydroxybutyronitrile using the same. The improved halohydrin epoxidase of the present invention consists of an amino acid sequence in which a specific amino acid substitution mutation is introduced into an amino acid sequence of a wild-type halohydrin epoxidase comprising predetermined amino acid sequences I and II.

Halohydrin epoxidase

An improved hydroxynitrile lyase characterized by having a mutation of substitution of at least one amino acid residue in the amino acid sequence of a wild-type hydroxynitrile lyase with another amino acid and by its hydroxynitrile lyase activity per transformant being higher than the hydroxynitrile lyase activity per transformant into which the wild-type hydroxynitrile lyase gene is introduced; and a method for producing a hydroxynitrile lyase, comprising expressing the improved hydroxynitrile lyase in a host and recovering the improved hydroxynitrile lyase from the resultant culture.

Hydroxynitrile lyase

Substitutional variants of hydroxynitrile lyase with high specific activity and methods of use

The present invention relates to an amide hydrolase which is with excellent thermostability and stereoselectively hydrolyzes an &#x3b1;-amino acid amide; a gene encoding the enzyme protein; a novel recombinant vector containing the gene; a transformant containing the recombinant vector; and a process for producing an L-&#x3b1;-amino acid using the transformant.

Polynucleotide encoding a thermostable amide hydrolase and methods for producing an L-&#x3b1;-amino acid

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Kanagawa, Japan

Takanori Akiyama