Arkray, Inc.

Takanari Shigemitsu

Naotsugu Onuma

Daisuke Matsumoto

Naoto Shichi

Jin Yoshida

A determination method includes: using a microchip, including a capillary flow path and a sample reservoir connected to the capillary flow path at an upstream side, to fill the capillary flow path with a first solution for electrophoresis, and supply the sample reservoir with a second solution containing an analyte; applying a voltage between the sample reservoir supplied with the second solution and the inside of the capillary flow path filled with the first solution, to move a component contained in the second solution in the capillary flow path and separate the component in the capillary flow path; optically detecting a value related to a component difference between the first solution and the second solution, other than a value related to the analyte, for the separated component; and determining whether the optical detection is favorable or poor by comparing the optically detected value with a predetermined threshold value.

Determination method, analysis method, and analysis system

A method of measuring stable A1c in a blood sample based on a time distribution of an optical measured value of hemoglobin at a flow path which separates hemoglobin in the blood sample on a basis of amounts of the charges of hemoglobin is provided. The method may include a step of obtaining a correction factor, based on a peak area (A) of a fraction including HbA0 and either a peak area (G) of a first fraction including chemically-modified HbA0, or a peak area (D) of a second fraction including a component having a smaller amount of positive charge than HbA0 adjacent to a fraction identified as HbA0, in the time distribution; and a step of correcting, based on the correction factor a peak area of a fraction including stable A1c in the time distribution.

Method of measuring stable A1c

A method of driving a pump is used in a pressure-applying apparatus, the apparatus including a flow passage, a pump configured to impart pressure into the flow passage, an opening and closing valve configured to open and close the flow passage, a pressure detector configured to detect pressure in the flow passage, and an atmospheric air open valve configured to open an interior of the flow passage to atmospheric air. The method includes driving the pump after closing the opening and closing valve and opening the atmospheric air open valve, and evaluating a state of the pump, based on one of: the pressure detected by the pressure detector at a time at which a predetermined time period has elapsed after closing the atmospheric air open valve, and a time from closing of the atmospheric air open valve until detection of a predetermined pressure by the pressure detector.

Method of driving an atmospheric air pump in a capillary electrophoresis apparatus

An analytical tool is provided for analysis of a sample by capillary electrophoresis. The analytical tool includes an inlet reservoir into which a sample is introduced, a capillary tube in communication with the inlet reservoir, a filter through which a liquid from the inlet reservoir passes, an enlarged portion undergoing a sudden increase in a cross-sectional area and being in communication with the inlet reservoir and the capillary tube, and a pressure fluctuation reducer for preventing pressure fluctuation at the enlarge portion from affecting a liquid in the capillary tube.

Analytical tool and analytical system

One embodiment of the invention provides: a microchip including a sample collection section and an analysis section, in which the sample collection section and the analysis section are imparted with both hydrophilicity and a positively-charged layer; an analysis system including the microchip; and a method of producing the microchip. The microchip includes: a sample collection section for collecting a sample; and an analysis section for analyzing the sample. In the microchip, a cationic polymer bonded with a hydrophilization substance is immobilized on inner walls of the sample collection section and the analysis section.

Microchip, analysis system, and method of producing microchip

A sample analysis method capable of improving separation accuracy of at least one of hemoglobin A2 and hemoglobin S and downsizing an analysis device is provided. The method includes separating hemoglobin in a sample in an alkaline solution containing a cationic polymer by capillary electrophoresis.

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Kyoto, Japan

Takanari Shigemitsu