Kyoto University

Osaka University

Takamichi Miyazaki

Hirofumi Suemori

Norio Nakatsuji

Kiyotoshi Sekiguchi

Eihachiro Kawase

Yukimasa Taniguchi

Sugiko Futaki

Maria Hayashi

Provided is a cell culture method comprising the step of culturing cells using a medium containing a laminin fragment having integrin binding activity, the method not comprising the step of coating a culture vessel with a laminin or a laminin fragment before seeding the cells in the culture vessel. The cell culture method of the present invention uses a smaller amount of a laminin fragment and still achieves a comparable culture efficiency as compared with the conventional cell culture method that uses a culture vessel precoated with a laminin or a laminin fragment.

Cell culture method using laminin fragment-containing medium

The present invention provides a culture substrate which enables maintenance culture of human pluripotent stem cells in a pluripotent state under a feeder-free culture environment, and a culture method of human pluripotent stem cells using the culture substrate. By seeding human pluripotent stem cells dissociated into single cells at a cell density of 4&#xd7;10to 10&#xd7;10cells/cmonto a culture substrate coated with human laminin &#x3b1;5&#x3b2;1&#x3b3;1 E8 fragment or human laminin &#x3b1;3&#x3b2;3&#x3b3;2 E8 fragment preferably at a concentration of 0.5 to 25 &#x3bc;g/cm, the human pluripotent stem cells can be rapidly expanded in a pluripotent state.

Culture substrate for human pluripotent stem cells and use thereof

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Kyoto, Japan

Takamichi Miyazaki