Daiichi Pure Chemicals Co., Ltd.

Takami Sarashina

Hiroyuki Ebinuma

Koji Ushizawa

Masahiro Sekiguchi

Takuji Matsumoto

The present invention provides a method for quantitatively determining homocysteine in a biological specimen containing homocysteine and cysteine by use of an enzyme which is capable of forming hydrogen sulfide both from homocysteine and from cysteine, which comprises (a) reacting the biological specimen with cysteine dioxygenase in the absence of a reducing agent, (b) subsequently reacting the resultant specimen of (a) with a reducing agent and the enzyme which is capable of forming hydrogen sulfide both from homocysteine and from cysteine, and (c) measuring the concentration of the hydrogen sulfide thus obtained to determine the homocysteine concentration in the biological specimen; and a reagent for such a quantitative determination of homocysteine.

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Naka-gun, Japan

Takami Sarashina