Research Institute of Innovative Technology for the Earth

Sumitomo Bakelite Company Limited

Takahisa Kogure

Masayuki Inui

Masako Suda

Kazumi Hiraga

Naoto Kato

Satoshi Hasegawa

Toru Jojima

Provided is a method for improving productivity in producing an organic compound in a bacterium that originally does not have an inherent ED pathway. In one aspect, provided is a transformant of a coryneform bacterium that is obtained by introducing the Entner-Doudoroff pathway into the coryneform bacterium as a host. In another aspect, provided is a transformant of a coryneform bacterium that is obtained by introducing, into a coryneform bacterium as a host a gene in which an enzyme having glucose-6-phosphate dehydrogenase activity is encoded, a gene in which an enzyme having 6-phosphogluconate dehydratase activity is encoded, and a gene in which an enzyme having 2-keto-3-deoxy-6-phosphogluconate aldolase activity is encoded.

Transformant having Entner-Doudoroff pathway and production method for organic compound using same

Provided is a microorganism that is able to efficiently produce protocatechuic acid or a salt thereof by using a saccharide as a raw material, and a method of efficiently producing protocatechuic acid or a salt thereof by using the microorganism. Provided is a transformant having protocatechuic acid producing ability, subjected to modifications of enhancement of 3-dehydroshikimate dehydratase activity; enhancement of chorismate pyruvate lyase activity; and enhancement of 4-hydroxybenzoate hydroxylase activity. Also provided is a method of producing protocatechuic acid or a salt thereof, including the step of culturing the transformant in a reaction solution containing a saccharide so as to cause the transformant to produce protocatechuic acid or a salt thereof.

Transformant, and method for producing protocatechuic acid or salt thereof using same

A coryneform bacterium transformant engineered by the following (A) to (D): (A) enhancement of 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase activity; (B) prevention, inhibition, or reduction of intracellular sugar uptake mediated by phosphotransferase system (PTS); (C) enhancement of intracellular sugar uptake activity mediated by a sugar transporter different from phosphotransferase system and enhancement of glucokinase activity; and (D) enhancement of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) activity is capable of efficiently producing shikimic acid or the like from a sugar.

Coryneform bacterium transformant and process for producing organic compound using the same

Growing community of inventors

Kyoto, Japan

Takahisa Kogure