Screen Holdings Co., Ltd.

Kyoto Prefectural Public University Corporation

Takahiro Tsujikawa

Hiroshi Ogi

Maki Hirai

Kyoko Itoh

Shunta Ishihara

An image processing using a plurality of specimen images obtained by successively applying a plurality of types of staining to a specimen to be evaluated and imaging the specimen after staining for at least two types of staining is performed. The image processing comprises: extracting an inner region corresponding to inward of a cell membrane of a single cell in the specimen based on at least one of the specimen images; specifying a cell region corresponding to an individual cell included in the specimen by expanding the inner region outwardly; and performing image cytometry for the cell region based on the specimen images. In analyzing a pathological specimen on a cell-by-cell basis, it is possible to deal with multiple immunostaining and specify the positions of individual cells and evaluate each cell separately even when a cell density in the specimen is high.

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Kyoto, Japan

Takahiro Tsujikawa