Panasonic Corporation

Matsushita Electric Industrial Co., Ltd.

Phc Holdings Corporation

Panasonic Healthcare Holdings Co., Ltd.

Takahiro Nakaminami

Shin Ikeda

Masaki Fujiwara

Shiro Nankai

Atsushi Fukunaga

Motokazu Watanabe

Shoji Miyazaki

Hiroyuki Tokunaga

Eriko Yamanishi

Yoshifumi Takahara

Junko Nakayama

Toshihiko Yoshioka

Tomohiro Yamamoto

Akihito Kamei

Miwa Hasegawa

Masato Suzuki

Jin Muraoka

Hiroaki Tachibana

Eiji Noguchi

Kouji Kashiwada

Takahiro Takasu

A reagent layer of a sensor contains as a mediator a quinone compound having a hydrophilic functional group, phenanthrenequinone, and/or a phenanthrenequinone derivative. The quinone compound has a lower redox potential than a conventional mediator, so interfering substances have less effect on detection results with this sensor.

Sensor and concentration measurement method

Voltage is applied across a counter electrode and a working electrode, with which a blood sample is in contact, in such a state that an oxidant in a redox substance is not substantially in contact with a working electrode but is in contact with a counter electrode and a reductant is not substantially in contact with the counter electrode but is in contact with the working electrode, whereby the reductant and the oxidant are respectively oxidized and reduced to measure current produced upon the oxidation and reduction. According to the above constitution, while lowering the voltage applied across the working electrode and the counter electrode, the Hct value of the blood sample can be measured stably with a satisfactory detection sensitivity. This measurement can be carried out with a sensor chip comprising a working electrode (), a counter electrode (), and a blood sample holding part () having branch parts (). A first reagent () consisting essentially of an oxidant in a redox substance is disposed in contact with the surface of a counter electrode which faces the branch part (), and a second reagent () consisting essentially of a reductant is disposed in contact with the surface of a working electrode that faces the branch part ().

Method for measuring hematocrit value of blood sample, method for measuring concentration of analyte in blood sample, sensor chip and sensor unit

Provided is a sensor chip for electrochemically measuring a concentration of an analyte in a blood sample. In one embodiment of the sensor, the sensor chip includes a substrate, and a preliminary measurement analyzer and a hematocrit value analyzer disposed on the substrate. The preliminary measurement analyzer includes a preliminary working electrode and a preliminary counter electrode. The hematocrit value analyzer includes a working electrode and a counter electrode. An oxidant of a redox substance is disposed on the preliminary measurement analyzer and the counter electrode. A reductant of a redox substance is disposed on the working electrode.

In a biosensor for measuring a specific substance in a liquid sample, one or a combination of sugar alcohol, metallic salt, organic acid or organic acid salt which has at least one carboxyl group in a molecule, and organic acid or organic acid salt which has at least one carboxyl group and one amino group in a molecule, is included in a reagent layer provided on electrodes, thereby providing a highly-accurate biosensor which is excellent in stability and has high response (sensitivity, linearity) of the sensor to the substrate concentration.

Biosensor

The measuring device of the invention includes: a first container and a second container for holding a sample; and an optical measurement part for carrying out an optical measurement. The first container has a first sample supply inlet for supplying a sample containing an analyte to the first container and at least one electrode. The second container has a second sample supply inlet for supplying the sample to the second container and a reagent holding part for holding a reagent for the optical measurement.

Measuring device, measuring apparatus and method of measuring

A measuring device () includes a base body () constituting: a sample holding portion () configured to hold a test sample containing urea and a material to be detected; and a sample supply port () through which the test sample is supplied to the sample holding portion (). The base body includes an optical measuring portion and a reagent holding portion () which are configured to carry out an optical measurement of the test sample held by the sample holding portion (). The reagent holding portion holds an antibody to the material to be detected and urease which causes hydrolysis of the urea. With this, the present invention provides a measuring device, a measuring apparatus, and a measuring method, each having a simple configuration and capable of reducing measurement errors caused by the urea contained in the test sample and accurately measuring the material to be detected.

Measuring device, measuring apparatus, and measuring method

A method for measuring the concentration of creatinine includes the steps of: (A) mixing a sample containing creatinine with a creatinine quantitative reagent including 1-methoxy-5-methylphenazinium in the absence of picric acid and any enzyme responsive to creatinine, to cause the creatinine to reduce the 1-methoxy-5-methylphenazinium; (B) electrochemically or optically measuring the amount of the 1-methoxy-5-methylphenazinium reduced in the step (A); and (C) determining the concentration of the creatinine contained in the sample from the amount of the reduced 1-methoxy-5-methylphenazinium measured in the step (B).

Method, device and apparatus for measuring the concentration of creatinine, and method, device and apparatus for measuring the amount of salt in urine using the same

A method for measuring a concentration of creatinine includes the steps of: (A) mixing a sample containing creatinine with a creatinine quantitative reagent containing a metal complex of at least one of hexacyanoferrate and hexacyanoruthenate in the absence of picric acid and any enzyme responsive to creatinine, to cause the creatinine to reduce the metal complex; (B) electrochemically or optically measuring the amount of the metal complex reduced in the step (A); and (C) determining the concentration of the creatinine contained in the sample from the amount of the reduced metal complex measured in the step (B).

Method, device and apparatus for measuring the concentration of creatinine, and method, device and apparatus for measuring the amount of salt using the same

A measuring device for analyzing an analyte contained in a sample. The device includes a hollow housing, a sample holding part provided inside the housing for holding the sample, a sample supply inlet provided for the housing so as to communicate with the sample holding part, an optical measurement part provided for the sample holding part for making an optical measurement, a reagent holding part provided for the sample holding part for holding a reagent for the optical measurement, and at least one electrode provided on an outer surface of the housing.

A measurement cellincludes: an opening partfor supplying a sample into a sample holding partfor holding a sample; an optical window portion for allowing light to enter the sample holding partand allowing light to exit the sample holding part; a protection coverfor protecting the optical window portion, provided along the circumference of the sample holding part; and a first protection-cover-holding partfor holding the protection coverat the position where the optical window portion is covered.

Growing community of inventors

Osaka, Japan

Takahiro Nakaminami