Toyo Seikan Group Holdings, Ltd.

Toyo Seikan Kaisha, Ltd.

Toyo Seikan Group Holdings Co., Ltd.

Takahiko Totani

Takashi Sato

Ryo Suenaga

Yoichi Ishizaki

Satoshi Tanaka

Yosuke Matsuoka

Kyohei Ota

Takeshi Aihara

Masahiro Kuninori

Takaharu Nishiyama

Osamu Koseki

Makoto Kobayashi

Ken Kashiwabara

Yousuke Matsuoka

Provided is a container for cell culture, provided with a container main body and injection/discharge ports, in which an inside of the container main body is divided into a plurality of cisternsst andnd by a filter member. Thus, various operations performed upon the cell culture can be simply and efficiently performed.

Container for cell culture and use method therefor

A cell culture method and a cell culture system, wherein: at least a part of a culture surface of a culture container is formed of a base material, the base material having a contact angle of not more than 84°, being selected from polyethylene, polypropylene, polymethylpentene, cyclic olefin polymer, cyclic olefin copolymer, polyvinyl chloride, polyurethane, methyl polymethacrylate, polyester, polyamide, ionomer, ethylene-vinyl acetate copolymer, ethylene-vinyl alcohol copolymer, ethylene-acrylic acid copolymer, ethylene-methyl acrylate copolymer, ethylene-methacrylic acid copolymer, ethylene-methyl methacrylate copolymer and the like, and having been irradiated or hydrophilized; a medium containing laminin or a fragment thereof as a cell adhesion factor and cells are accommodated in the culture container; and adherent cells are thus cultured so that the adherent cells can be preferably cultured without coating the culture container with laminin.

Cell culture method and cell culture system

A culture container for culturing lymphocytes includes an immobilized surface and a non-immobilized surface, wherein the culture container is formed of a gas permeable film, the immobilized surface and the non-immobilized surface are container inner surfaces facing each other, and anti-CD3 antibodies are immobilized in the immobilized surface at a concentration of 10 to 300 ng/cm.

Culture container, method for culturing lymphocytes, culture-container production method, and solid-phasing apparatus

In the production of a culture vessel in which a low cell-adhesive polymer as a coating agent is applied to a culture surface having a plurality of concave parts formed thereon, a use amount of the coating agent can be reduced. The low cell-adhesive polymer as the coating agent is applied to a surface of a culture base material in advance, and subsequently the culture base material is subjected to processing to form the culture vessel having the surface of the culture base material coated with the coating agent as the culture surface.

Method for producing culture vessel, and culture vessel

A culture container made of a flexible packaging material where the culture container includes a first side surface portion and a second side surface portion that are mutually opposed. The culture container also includes at least an inner surface side of the first side surface portion which has a surface treatment such that, when a contact angle hysteresis is defined as an advance contact angle—a retreat contact angle at a time when water slips, a contact angle hysteresis of a surface on the inner surface side of the first side surface portion becomes larger than a contact angle hysteresis of a surface on an inner surface side of the second side surface portion. The culture container further includes a membrane made of a cell adhesion inhibitor that is formed on the inner surface side of the first side surface portion.

Culture container, method for manufacturing culture container, laminated structure, and method for manufacturing laminated structure

A vessel manufacturing method includes placing a bag-shaped, film-based vessel on a placement stage with concave portions formed on the stage, introducing fluid into the vessel placed on the stage, and pressing the vessel which is placed on the stage, by a pressing member while heating at least one of the stage and the pressing member. A cell culture vessel is also provided which includes a first vessel wall as a bottom wall and a second vessel wall. The first vessel wall is formed of a flat film having gas permeability. The second vessel wall is disposed in contact with a peripheral edge portion of the first vessel wall, and has a bulge shape protruding relative to the first vessel wall. The first vessel wall is flat at its section other than a region where the first vessel wall is in contact with at least a charge/discharge port.

Method and apparatus for producing container, cell culture vessel, method for culturing cells, method for producing cell culture vessel, and apparatus for producing cell culture vessel

A culture container made of a flexible packaging material where the culture container includes a first side surface portion and a second side surface portion that are mutually opposed. The culture container also includes at least an inner surface side of the first side surface portion which has a surface treatment such that, when a contact angle hysteresis is defined as an advance contact angle-a retreat contact angle at a time when water slips, a contact angle hysteresis of a surface on the inner surface side of the first side surface portion becomes larger than a contact angle hysteresis of a surface on an inner surface side of the second side surface portion. The culture container further includes a membrane made of a cell adhesion inhibitor that is formed on the inner surface side of the first side surface portion.

Provided is a culture container base material made of a polyolefin material, capable of readily forming a culture container for culturing adherent cells. The culture container base material is for culturing the adherent cells and made of the polyolefin material. At least a part of a surface of the base material is subjected to a surface treatment, where the surface of the base material is a culture surface of the culture container, and the surface subjected to the surface treatment has a static water contact angle of greater than 80° and a receding contact angle of less than 53°.

Culture container base material, culture container, and production method of culture container base material

Provided are a multilayer film having excellent gas permeability and excellent handling properties and hence is suited for forming a cell culture container, and a cell culture container formed by using the same. A multilayer film used for forming a cell culture container, comprising: a base material composed of a polyethylene-based resin having a density of 0.87 g/cmto 0.90 g/cm; and an inner layer composed of a polyethylene-based resin having a density of 0.896 g/cmto 0.93 g/cmand forming a cell culture icy surface. A cell culture container is formed by using this multilayer film.

Culturing cells in a multilayer film container

A cell culture container includes a container main body comprising a gas-permeable plastic film, and an injection/discharge port. A peripheral portion of the container main body is sealed, and the container main body has a bulging shape on a top surface side of the container main body. A plurality of concave portions serving as cell culture parts are provided on a bottom surface of the container main body.

Cell culture vessel, support jig for cell culture vessel and cell culture method

A culture container for activating lymphocytes includes immobilized anti-CD3 antibodies and an anti-CD3 antibody solution including anti-CD3 antibodies, wherein the culture container is formed in a bag-like shape and is formed of a soft-packaging material, the immobilized anti-CD3 antibodies are immobilized at a density of 10 to 300 ng/cmon one surface of opposing inner surfaces within the container, and the anti-CD3 antibody solution is enclosed in the container in an amount of 0.25 to 400 ng of the anti-CD3 antibodies in 0.1 to 800 &#x3bc;l of the solution per 1 cmof a culture surface formed of the one surface.

Culture container and method for manufacturing culture container

A culture bag for culturing adherent cells is provided without requiring a highly clean production environment and a complex production step such as a masking step. An adherent cell culture vessel that is formed of a polyolefin is configured so that part or the entirety of the inner surface of the culture vessel has a static water contact angle of 95&#xb0; or more, and has an advancing contact angle and a receding contact angle that satisfy the inequality 'advancing contact angle&#x2212;receding contact angle&#x3e;25&#xb0;' when water runs down along the inner surface.

Culture container for adherent cells and method for producing culture container for adherent cells

A cell culture kit for cultivating cells in a closed system includes at least one or more of each of: a culture container for cultivating cells; a culture medium storage container for storing a culture medium or the like; a cell injection container for injecting cells; and a cell collecting container for collecting a suspension of cells after cultivation; wherein the culture medium storage container is also used as a waste liquid container for collecting a culture medium after cultivation, and the culture container, the culture medium storage container, the cell injection container and the cell collecting container are linked to one another through a conduit.

Cell culture kit, and method of using cell culture kit

Adhesive culture cells cultured by using a culture container made of a flexible film can selectively be released from the culture container without causing damage to the cells. A cell release method for releasing, from a culture container, adhesive culture cells cultured adhering to the culture container made of a flexible film includes bringing a surface of the film of the culture container into contact with the adhesive culture cells, applying a pressure to the adhesive culture cells, and releasing the adhesive culture cells from the culture container, wherein the surface of the film faces a surface of the film of the culture container to which the adhesive culture cells adhere.

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Kanagawa, Japan

Takahiko Totani