Tosoh Corporation

Tosch Corporation

Takahiko Ishiguro

Juichi Saitoh

Toshiki Taya

Kiyoshi Yasukawa

Toshinori Hayashi

Tetsuya Ishizuka

Juichi Saito

Ryuichi Horie

Noriyoshi Masuda

Takahiro Maruyama

Takumi Tokunaga

Shigeo Tsuchiya

Masami Otsuka

Akihiro Yokoyama

Teruhiko Inoue

Yukio Sugiura

Yoshifumi Kurihara

Takashi Yamamoto

Shingo Fukunaga

Takao Matsuba

Yasutami Mitoma

Toshitaka Taya

Hideo Yawata

An olignucleotide capable of binding to the intramolecular structure-free region of Verotoxin type 1 RNA or Verotoxin type 2 RNA at relatively low and constant temperature, and which can be used in a constant temperature nucleic acid amplification method, is provided. Also, a simple, speedy and highly sensitive method for detecting Verotoxin type 1 RNA or Verotoxin type 2 RNA is provided.

Oligonucleotide and method for detecting Verotoxin

Nucleic acid sequences, oligonucleotides and a method for detection of SRSV, in particular, a virus which belongs to Genotype II (GII), in clinical examinations, public health examinations, food evaluations and food poisoning examinations are provided.

Oligonucleotides and method for characterizing and detecting Genogroup II type small round structured virus

An oligonucleotide for cleavage, detection or amplification of the mecA gene, a gene element of methicillin-resistant(MRSA), or RNA derived from said gene is provided. Further, a method for detecting the mecA gene is provided.

Oligonucleotides and method for detection of meca gene of methicillin-resistant Staphylococcus aureus

A simple and accurate method for assay of a single-stranded RNA containing a specific nucleic acids sequence in a sample at almost constant temperature by using at least the following reagents (A) to (I), which comprises a step of adding the reagents (A) to (I) one by one (in any order), in combinations of at least two or all at once and

Method of assay of target nucleic acid

An oligonucleotide, capable of specific cleavage and amplification of a pab gene which codes for pab, an antigenic protein of, or an RNA derived therefrom, as well as being useful in high sensitive detection and identification thereof, is provided. Further, a combination of oligonucleotides, useful in specific amplification, high sensitive detection and identification of an RNA derived frompab gene as well as a process for detectingby using said combination, are provided.

Oligonucleotides for detecting tubercle bacillus and method therefor

A method of assaying the efficacy and/or toxicity of a test substance by expression of a specific gene in a cell or a microorganism, which comprises treating the cell or the microorganism with the test substance, a step of amplifying an RNA having a sequence homologous or complementary to a specific sequence in a target RNA obtained as the result of transcription of the specific gene, and a step of determining whether the target RNA is transcribed through the expression of the specific gene by detecting the RNA amplified in the previous amplification step.

Method of evaluating drug efficacy and toxicity

An oligonucleotide useful for detection of an RNA derived from HIV-1 consisting of at least 10 consecutive bases in any of SEQ ID NOS:1, 2, 4 to 10 and 13 to 17, which can bind to a specific site of the RNA.

Oligonucleotide for detection of HIV-1 and detection method

The present invention provides oligonucleotides for detectingtoxin gene invA mRNA and stn mRNA which oligonucleotides specifically bind to invA mRNA or stn mRNA at a relatively low temperature (for example, 41&#xb0; C.) and at a constant temperature, and a process of amplifyingtoxin gene invA mRNA or stn mRNA and a method of detecting the same using the oligonucleotides.

Oligonucleotide for detecting Salmonella and method of detecting Salmonella

A simple, speedy and sensitive method of detecting HIV-RNA using an oligonucleotide which can bind to an intramolecularly free region of the genomic RNA of HIV-1 at relatively low and constant temperatures (at 35&#xb0; C. to 50&#xb0; C., preferably at 41&#xb0; C.) as an oligonucleotide primer for use in amplification of a nucleic acid.

Method of amplifying or detecting HIV-1 RNA

An oligonucleotide useful for specific amplification of HCV RNA, or highly sensitive detection and identification of HCV RNA, and a combination thereof. A detection method using RNA amplification step, wherein the oligonucleotide of SEQ ID NO: 11 is used as a first primer and the oligonucleotide of SEQ ID NO: 6 or 7 as a second primer, the oligonucleotide of SEQ ID NO: 12 is used as a first primer and the oligonucleotide of SEQ ID NO: 7 as a second primer, or the oligonucleotide of SEQ ID NO: 13 is used as a first primer and the oligonucleotide of SEQ ID NO: 9 as a second primer.

Oligonucleotide for highly sensitive detection of hepatitis C virus and method for detection thereof

A compound represented by the formula 1, a salt, hydrate, solvate or stereoisomer thereof.

Fluorescent dye and method of measuring nucleic acid

A method for detecting fluorescence from a liquid sample housed in a transparent or translucent sample container includes providing a sample container in a sample holder, which is opaque except for a sample container introduction opening and incoming and outgoing openings for excitation light; layering a liquid sample and a shielding liquid unmixable therewith to prevent external light from entering through the sample container introduction opening; introducing excitation light from such a direction that the excitation light can irradiate the liquid sample before irradiating the shielding liquid; and detecting fluorescence, which emits in a direction of avoiding absorption by the shielding liquid.

Fluorescence detection method capable of making measurement under external light

Nucleic acid probe

An oligonucleotide for detection or amplification of a gene selected from the group consisting of thermostable direct hemolysin-related hemolysin genes (trh1 and trh2) and thermostable direct hemolysin gene (tdh2) or RNA derived therefrom is provided. Further, method for detecting trh1, trh2 or tdh2 using said oligonucleotide is provided.

Growing community of inventors

Yokohama, Japan

Takahiko Ishiguro