Hitachi, Ltd.

Hitachi High-tech Corporation

Hitachi-high-technologies Corporation

Takahide Yokoi

Takashi Anazawa

Chihiro Uematsu

Yusuke Goto

Yoshimitsu Yanagawa

Itaru Yanagi

Makiko Yoshida

Mima Ogawa

Riichiro Takemura

Takayuki Kawahara

Yohei Kawaguchi

Masao Kamahori

Masataka Shirai

Naoshi Itabashi

Yoshiaki Yazawa

Michiru Fujioka

Kazuma Matsui

Yoshinobu Kohara

Rena Akahori

Junko Tanaka

Tomohiro Yasuda

Yuichi Uchiho

Takamichi Muramatsu

Sonoko Migitaka

George Chalkidis

An electrophoresis device is used to separate a biological substance using a separation medium. A recovery hole configured to recover the separated target biological substance is formed in an electrophoresis device, the recovery hole is configured to be capable of accommodating a solvent that can suspend the target biological substance, a buffer tank configured to accommodate a buffer solution is provided below the recovery hole, and a membrane is disposed between the recovery hole and the buffer tank.

Electrophoresis device and biological substance recovery method

The present disclosure proposes a probe including a sequence recognition portion that is capable of specifically binding to a desired DNA sequence, and a first probe portion that includes a plurality of 3-dimensional structures arranged downstream from an end of the sequence recognition portion, in which the plurality of 3-dimensional structures are arranged in an order so that the order corresponds one-to-one with the DNA sequence.

Probe, probe set, and method for identifying desired DNA sequence in solution

The present invention optically obtains characteristic values of a plurality of biological samples from a specimen; compares the characteristic values with examination information associated with the same specimen obtained in advance; and calculates an indicator to determine, based on a result of the comparison, whether a process is continued to genetic analysis of the biological samples or a re-preparation of samples from the same specimen is performed. By this method, it is possible to achieve more reliable biomarker detection by ensuring validity through appropriate selection of test samples while reducing cost, effort, and time for analysis in single cell analysis or analysis of a group of a small number of cells.

Examination system, examination device, and examination method for testing sample quality prior to biomarker detection

Efficient simultaneous laser-irradiation-fluorescence detection is performed for a plurality of channels of a microchip and simple and highly sensitive parallel analysis of a plurality of samples is enabled. In a microchipmade of an optically transparent solid material mwith a refractive index n, a plurality of channelsfilled with a material mwith a refractive index nand a plurality of channelsfilled with a material mwith a refractive index nare alternately arranged in parallel on the same plane. Here, m, mand mare selected such that a relation of n<n<nis satisfied. The material mis an optically transparent liquid suitable for targeted analysis. When a laser beamis focused and irradiated perpendicularly to the individual channelsandalong the same plane, refraction of the laser beamby the channelsand refraction of the laser beamby the channelsare counterbalanced. For this reason, the laser beampropagates linearly on the same plane and the individual channelsandcan be simultaneously and efficiently irradiated with the laser beam. Fluorescence emitted from the individual channelsandis detected independently and simultaneously using a collimating lens, a filter and a diffraction grating, an imaging lens, a two-dimensional sensor, and a data analysis system, from a direction perpendicular to the same plane.

Microchip and manufacturing method therefor, and multi-channel fluorescence detection device

Provided is a technique for easily trapping microbiota in a sample. A microbiota trapping device of the present disclosure includes a first solution tank having a first filter adapted to trap residue in a sample, and a second solution tank having a second filter adapted to trap bacteria in the sample. The second solution tank has a vent above the second filter.

Microbiota trapping device, microbiota trapping apparatus, and microbiota trapping method

The present invention is directed to provide new PCR measuring method and device. As one embodiment of the present invention, a DNA detection method for detecting DNA in a droplet being present in oil, the droplet containing the DNA and a fluorescent labeled probe, the fluorescent labeled probe being hybridized to the DNA, the method including: a first step of amplifying the DNA in the droplet by a nucleic acid amplification reaction; and a second step of measuring a melting temperature of the fluorescent labeled probe and the DNA in the droplet is provided.

PCR measuring method and measurement device

An electrophoresis method, system, and gel recover biological substances from the gel with high efficiency. The method uses an electrophoresis gel having an injection hole into which biological substances are injected and a recovery hole from which the biological substances are recovered. The electrophoresis method includes injecting the biological substances into the injection hole, and applying an electric field penetrating the injection and recovery holes. A vertical axis in a downward direction as a positive direction is set as an X-axis, an axis which is perpendicular to the X-axis is set as a Y-axis, and coordinates of a bottom of the recovery hole are set as (X, Y). The X coordinate Xof the bottom of the recovery hole satisfies X&#x3e;Xwhen the biological substance is electrophoresed to coordinates (X, Y) in the recovery hole from a bottom of the injection hole in the applying the electric field.

Electrophoresis method, electrophoresis system, and electrophoresis gel

Provided are a first tank; a second tank; a thin film having a nanopore, which communicates the first tank to the second tank, and disposed between the first and second tanks; a first electrode provided in the first tank; and a second electrode provided in the second tank. A wall surface of the nanopore has an ion adsorption preventing structure to prevent desorption/adsorption of an ion contained in a solution filling the first tank and/or the second tank, and a voltage is applied between the first and second electrodes to measure an ion current flowing through the nanopore.

Current measurement device and current measurement method using nanopore

A base sequence determination apparatus includes (1) a mobility correction unit that outputs a mobility correction signal obtained by mobility correction of a time-series signal of a wavelength spectrum corresponding to each base, (2) a deconvolution unit that executes processes for calculating a deconvoluted signal of the mobility correction signal respectively for a plurality of parameter candidates of point spread function, calculating variance of peak intervals for the calculated deconvoluted signal, specifying a parameter of the point spread function using the calculated variance, and outputting the deconvoluted signal corresponding to the point spread function having the specified parameter as an updated deconvoluted signal, (3) a peak extracting unit that extracts a peak waveform from the updated deconvoluted signal and outputs an updated peak-extracted signal, and (4) a sequence specifying unit that inputs the updated peak-extracted signal and determines a base sequence.

Base sequence determination apparatus, capillary array electrophoresis apparatus, and method

An object of the present invention is to solve inhibition of biopolymer measurement in a nanopore, which involves three-dimensional conformation of a biopolymer containing a nucleic acid. The present invention provides a device for analyzing a biopolymer containing a nucleic acid, the device including: an array device including a plurality of thin film parts having a nanopore; a single common container and a plurality of individual containers capable of storing a measurement solution which is brought into contact with the thin films; and individual electrodes respectively provided in the plurality of individual containers, wherein: the measurement solution is introduced into each of the individual containers and the common container so as to be brought into contact with the thin films; and the measurement solution has a pH equal to or greater than pKa of a guanine base and contains cesium ions as electrolyte cations.

Measuring reagent and analysis device for analyzing biopolymer

Conventionally, only a pair of electrodes is provided and nanopores arranged in parallel are connected by an electrolyte solution, and therefore a change in an ion current to be measured is a sum of changes in ion currents generated in the respective nanopores. The invention includes: a first solution chamber including a plurality of first electrodes; a second solution chamber including a second electrode which is a counter electrode of the first electrodes; a membrane provided between the first solution chamber and the second solution chamber, the membrane having a plurality of small holes; a measuring unit for applying a voltage between the first electrodes and the second electrode and measuring a current flowing between the first electrodes and the second electrode via the small holes; and an insulating structure forming unit for forming, in the first solution chamber, an insulating structure for insulating the first electrodes in a state in which a conductive liquid is filled between the small holes and the first electrodes and achieves parallel measurement of ion currents by using nanopores.

Current measuring device, current measuring method, and current measuring kit

Provided is a biomolecule measuring device capable of effectively reducing measurement noise occurring when measuring a biomolecule sample using a semiconductor sensor. This biomolecule measuring device generates a trigger to react a sample with a reagent after starting to send the reagent onto the semiconductor sensor that detects ion concentration (see FIG.).

Biomolecule measuring device

An aspect of the present invention includes performing electrophoresis of a nucleic acid sample to be analyzed labeled for each base type, generating waveform data of detected intensity by detecting a label signal for the each base type, selecting another peak position for each peak position of the waveform data for each base type, calculating relative signal intensity of signal intensity in each position relative to the signal intensity in the other selected position, and analyzing existence of each base type in a base sequence coordinate position of the nucleic acid sample by comparing the relative signal intensity of the nucleic acid sample to be analyzed and the relative signal intensity of a known nucleic acid sample in each peak position. Accordingly, acquiring information about a gene mutation in trace amounts existing in a target gene region highly sensitively with high precision is realized.

Gene mutation analyzer, gene mutation analysis system, and gene mutation analysis method

In the field of the next generation DNA sequencer, a method for integrating very high sensitive FET sensors having side gates and nanopores as devices used for identifying four kinds of base and for mapping the base sequence of DNA without using reagents, and a semiconductor device having selection transistors and amplifier transistors respectively corresponding to the FET sensors having side gates and nanopores respectively so as to be able to read the variation of a detection current based on the differences among the charges of the four kinds of base without deteriorating the detection sensitivity of the FET sensor, are presented.

Semiconductor device and manufacturing method thereof

A nucleic acid amplification method includes ligating a double-stranded adapter () containing adapter DNA strands capable of forming a folded structure to a double-stranded DNA () containing a target DNA sequence () to prepare a cyclic DNA template composed of double-stranded DNA containing a nick (). A 3'-end elongation reaction is performed using a strand-displacement DNA polymerase from the nick () as an origin, thereby producing a concatemer () in which a plurality of the target DNA sequences () and the adapter DNA strands capable of forming the folded structure are linked in series as a single-stranded DNA. The concatemer () contains a plurality of the target DNA sequences () suitable for nucleotide sequence analysis and has a folded shape such that it takes the form of a ball due to its folded structure.

Growing community of inventors

Tokyo, Japan

Takahide Yokoi