Toyo Seikan Group Holdings, Ltd.

Takaharu Nishiyama

Takashi Sato

Yosuke Matsuoka

Takahiko Totani

Osamu Koseki

An adherent cell culture substrate, which is a sheet-shaped substrate used for producing a culture vessel for adherent cells, includes a groove including a crest-shaped portion and a valley-shaped portion on one surface side of the substrate, in which a distance from a top end portion of the crest-shaped portion to the other surface of the substrate is 1 mm or less. Further, it is preferable that a cross section of the groove perpendicular to a direction in which the groove extends is formed in a substantially V-shape and an inclination angle of a side surface of the substantially V-shape of the groove is 80 degrees or less. Further, it is preferable that a plurality of the grooves are linearly arranged in parallel and the top end portions of the crest-shaped portions are formed in a linear shape.

Adherent cell culture substrate, culture vessel, cell detachment method, and method for producing adherent cell culture substrate

In the production of a culture vessel in which a low cell-adhesive polymer as a coating agent is applied to a culture surface having a plurality of concave parts formed thereon, a use amount of the coating agent can be reduced. The low cell-adhesive polymer as the coating agent is applied to a surface of a culture base material in advance, and subsequently the culture base material is subjected to processing to form the culture vessel having the surface of the culture base material coated with the coating agent as the culture surface.

Method for producing culture vessel, and culture vessel

Provided is a culture container base material made of a polyolefin material, capable of readily forming a culture container for culturing adherent cells. The culture container base material is for culturing the adherent cells and made of the polyolefin material. At least a part of a surface of the base material is subjected to a surface treatment, where the surface of the base material is a culture surface of the culture container, and the surface subjected to the surface treatment has a static water contact angle of greater than 80° and a receding contact angle of less than 53°.

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Kanagawa, Japan

Takaharu Nishiyama