Teijin Limited

Takaharu Kubota

Kenji Hosoda

Hideaki Suzuki

Hitomi Honda

Yasuhiko Masuho

Kiyoshi Nawata

Yuji Fukumoto

A method of immunologically assaying intact human osteocalcin in a human assay sample is provided by using an antibody having an epitope in a region of an amino acid sequence 1 to 20 on the N-terminal side of human osteocalcinand an antibody having an epitope in a region of an amino acid sequence 36 to 49 on the C-terminal side of human osteocalcin. Furthermore, a method of immunologically assaying the total amount of human intact osteocalcin in a human assay sample is provided. A reagent and a kit therefor are also described, as well as a monclonal antibody and a polyclonal antibody used for the assay a process for producing these antibodies; and a process for use of these antibodies.

Method of immunological assaying human osteocalcin, reagent and kit

A method of immunologically assaying intact human osteocalcin in a human assay sample is provided by using an antibody having an epitope in a region of an amino acid sequence 1 to 20 on the N-terminal side of human osteocalcin and an antibody having an epitope in a region of an amino acid sequence 36 to 49 on the C-terminal side of human osteocalcin. A reagent and a kit therefor are provided. Furthermore, a method of immunologically assaying the total amount of human intact osteocalcin in a human assay sample, and a reagent and a kit therefor are also provided. A monoclonal antibody and a polyclonal antibody are used for the assay. A process for producing these antibodies, and utilization of these antibodies is described.

Method of immunological assaying of human osteocalcin, reagent and kit

A method of immunologically determining human acid glutathione S-transferase in a human assay sample, which comprises bringing the assay sample into contact with a first antibody bound to an insoluble solid carrier and a labelled second antibody, either the first or second antibody being a polyclonal antibody capable of recognizing human acid glutathione S-transferase or an equivalent fragment of the polyclonal antibody, and the other antibody being a monoclonal antibody capable of specifically recognizing human acid glutathione S-transferase or an equivalent fragment of the monoclonal antibody, a method of diagnosing cancer in a human digestive organ by using the above method, a reagent therefor, a kit therefor and a monoclonal antibody for use therein.

Method of determining human acid glutathione S-transferase, reagent,

An immunoassay method performed in solution wherein a protein having an average molecular weight of 16,000 to 50,000 and an isoelectric point of 1.0 to 5.0 or a mixture containing the same is permitted to exist as the antigen-antibody reaction controller in the immunoreaction solution, and the final concentration of the antigen-antibody controller in the immunoreaction solution is controlled to 0.02 to 0.9% by weight, and a reagent kit to be used for an immunoassay containing the above antigen-antibody reaction controller at a final concentration of 0.02 to 0.9% by weight in the immunoreaction solution, as a part of the constituents thereof.

Immunoassay and reagent kit used therefor

A reagent for measuring immune complexes fixed with an anti-C3 antibody Facb fragment. By using the reagent, immune complexes existing in the blood serum or body fluid are quantitatively measured.

Reagents for measurement of immune complexes and method for measurement

A standard material for measuring immune complexes which is prepared by chemically binding immunoglobulin and/or its fragment with a complement and/or its derivative through the medium of a bifunctional reagent. By using the standard material, the standard curve which is used for measuring immune complexes existing in the blood is obtained.

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Hino, Japan

Takaharu Kubota