Seikagaku Corporation

Takaaki Kobayashi

Takashi Muramatsu

Hisako Muramatsu

Itsuo Yokoyama

Haruko Ogawa

A full-length cDNA is obtained by analyzing the partial amino acid sequence of purified endo-&#x3b2;-galactosidase C, constructing primers based thereon, effecting PCR with the use of the genomic DNA ofas a template to thereby obtain a fragment of cDNA encoding endo-&#x3b2;-galactosidase, effecting cassette PCR by using the cDNA fragment thus obtained to thereby obtain the 5'-terminal and 3&#x2032;-terminal domains, and further effecting PCR with the use of primers constructed on the basis of the data acquired above.

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Nagoya, Japan

Takaaki Kobayashi