Tanabe Seiyaku Company, Limited

Daicel Chemical Industries, Ltd.

Taizo Watanabe

Tetsuya Tosa

Satoshi Minobe

Ichiro Chibata

Tadashi Sato

Takao Mori

Takeji Shibatani

Masahiro Kagotani

Zenjiro Honda

Shinji Nagamatsu

Motoki Fujimura

Makoto Masuda

Yuhsi Matuo

Tomonobu Ase

Takashi Nishimura

Ryujiro Sano

There is disclosed a method for determining an endotoxin by reacting the endotoxin adsorbed on an adsorbent having specific endotoxin adsorbability with an endotoxin detecting reagent, including the steps of:

Method for determining endotoxin and apparatus therefor

A method for determination of a pyrogen content which comprises bringing a specimen into contact with an adsorbent composed of a nitrogen-containing heterocyclic compound bonded to a water-insoluble carrier directly or through a spacer, and after or without eluting the pyrogen adsorbed on the adsorbent, determining the content of the adsorbed pyrogen by the Limulus method.

Method for determining pyrogen content

Pyrogens are removed out of a solution thereof by (1) treating the solution with a membrane and then (2) treating the resulting solution with a pyrogen adsorbent. The obtained solution is useful in pharmaceutical fields.

Process for removing pyrogens

A method for removing a pyrogen from a solution of a pyrogen-containing substance such as an amino acid, a nucleic acid base, an antibiotic, a hormone, a vitamin, an enzyme, an antibody or the like comprising contacting the solution with an adsorbent to adsorb the pyrogen, which is characterized in that the adsorbent comprises a water-insoluble carrier and a nitrogen-containing heterocyclic compound of the formula:

Method for reducing the pyrogen content of or removing pyrogens from

An immobilized aminoacylase preparation is obtained by adsorbing tannin on a water-insoluble, hydrophilic carrier to give a water-insoluble tannin preparation, adsorbing aminoacylase on the tannin preparation to give a water-insoluble tannin-aminoacylase preparation, and then binding ferric or ferrous ion thereto. The immobilized preparation obtained above sustains a high rate of enzymatic activity for a long period of time when used in an enzymatic reaction thereof with N-acyl-DL-amino acids.

Immobilized aminoacylase

A water-insoluble tannin preparation is obtained by covalent binding or physical adsorption of tannin onto a water-insoluble, hydrophilic carrier. The preparation has a specific affinity for proteins and can be used as an adsorbent for purification, isolation and/or separation of proteins (e.g., Enzymes, albumin, globulin, hormonal proteins) from a mixture of compounds. Further, the water-insoluble tannin preparation having a catalytically active enzyme absorbed thereon can be used as a heterogeneous catalyst to induce enzymatic reactions.

Water-insoluble tannin preparation for immobilization of proteins

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Nagaokakyo, Japan

Taizo Watanabe