Nissan Chemical Industries Limited

Nissan Chemical Corporation

Kyoto University

Reprocell, Inc.

Chiba University

The University of Toyama

Institute of National Colleges of Technology, Japan

The University of Tokyo

National Institute of Technology

Taito Nishino

Ayako Otani

Takahiro Kishioka

Yoshiomi Hiroi

Koichiro Saruhashi

Takehisa Iwama

Tatsuro Kanaki

Ayako Aihara

Shunsuke Iwamoto

Masato Horikawa

Makiko Umezaki

Misayo Tomura

Tomoyuki Ozawa

Norio Nakatsuji

Norihisa Ishiwata

Natsuki Abe

Tomomi Otsuji

Yasuyuki Asai

Makiko Yui

Misayo Otani

Masao Yamamoto

Masaki Kitahara

Koji Eto

Katsuaki Miyaji

Hiromitsu Nakauchi

Yuki Usui

Takanori Nakamura

Daisuke Sakuma

Hiroyuki Nakajima

Tomoya Ohashi

Hisato Hayashi

Shigeo Kimura

Keigo Matsumoto

Hiromi Kitano

Kenichiro Kamei

Tadashi Nakaji

Hiroharu Kawahara

Yong Chen

Takumi Mikashima

Junko Katayama

Li Liu

Atsushi Iwama

Atsushi Miyamura

Yo Kishikawa

Hisao Kamiya

Miya Hiroi

Yuki Ueda

Hisashi Ishii

Masahiro Kamaura

Keiichiro Otsuka

Kayoko Tsuji

The invention provides a composition for forming a coating film containing a phosphoric acid group-containing polymer capable of forming a film on a substrate by a simple process and capable of maintaining film properties under a solvent environment containing water, and a coating film which is a cured product of the composition. In particular, the composition for forming a coating film contains (a) a polymer (P) containing a hydroxyl group, and the hydroxyl group is derived only from a phosphoric acid group, (b) a polycarbodiimide (C) containing a structure represented by the following formula (c-1):—N═C═N—  (c-1),and (c) a solvent.

Composition for forming coating film containing phosphoric acid group-containing polymer

Provided are a ligand-bearing substrate which has a surface at least partially coated with a polymer (P3) containing structural units represented by the formulae (1) and (1) (in the formulae, R, R, X, Y, L, Q, Q, Q, m1, m2 and n are as described in the claims and description); a raw material for such a substrate; and a method for producing such substrates.

Substrates coated with selective cell separation or cell culture polymers

An object of the present invention is to provide a cryopreservation vessel which has a coating that is capable of inhibiting the adhesion of cells or proteins and is resistant not only to aqueous solvents but also to organic solvents (for example, cryoprotectants, in particular, dimethyl sulfoxide). The invention provides a cell or protein cryopreservation vessel including, on at least a portion of the surface thereof, a coating that includes a repeating unit containing a group represented by the following formula (a), and a repeating unit containing a group represented by the following formula (b) (wherein U, U, U, U, U, Uand Anare as defined in the description and the claims).

Cryopreservation vessel

The present invention provides a culture method of cells and/or tissues including culturing cells and/or tissues in a suspended state by using a medium composition wherein indeterminate structures are formed in a liquid medium, the structures are uniformly dispersed in the solution and substantially retain the cells and/or tissues without substantially increasing the viscosity of the solution, thus affording an effect of preventing sedimentation thereof, and the like.

Culture medium composition and method of culturing cell or tissue using thereof

This is to provide a coating film having a function of inhibiting adhesion of a biological substance, a method for manufacturing the coating film, a copolymer obtainable by polymerizing a specific monomer mixture, a composition for forming a coating film having a specific composition, a method for manufacturing a varnish containing a copolymer to be used as a raw material of the composition for forming a coating film which is used for forming said film, and a sol for forming the coating film. In particular, this is to provide a coating film obtained by the method comprising a process of applying a composition for forming a coating film which comprises a copolymer comprising a recurring unit containing an organic group of the formula (a) and a recurring unit containing an organic group of the formula (b) and a solvent onto a substrate; and a process of drying at a temperature of &#x2212;200&#xb0; C. to 200&#xb0; C. (wherein U, U, U, Uand U, and Anare as defined in the present specification and the claims). 

Ion complex material having function of inhibiting adhesion of biological substance and method for manufacturing the same

The invention provides a medium additive, medium composition and a culture method and the like, capable of efficiently culturing cells or tissues in a well dispersed state, and further, permitting cell image analysis of the cells or tissues. The medium additive or medium composition contains agar, which preferably is a low molecular weight agar having a weight average molecular weight of 10,000-60,000. Using same, cells or tissues can be cultured in a well-dispersed state in a medium, and a proliferation promoting effect for the cells or tissues can also be obtained. In addition, the cells can be cultured in any of a floating state and a precipitated state by adjusting the concentration of the aforementioned agar.

Culture medium additive, culture medium composition, and method for culturing cells or tissue using same

The invention provides a coating agent for a flow passage which comprises a copolymer containing a recurring unit which contains an organic group of the formula (a) wherein Uand Uare defined herein, and a recurring unit which contains an organic group of the formula (b) wherein U, U, U, and Anare defined herein. The invention also provides a flow passage device having the coating agent for a flow passage on at least part of an inner surface of a flow passage, a platelet-producing flow passage device and a method for manufacturing the same.

Coating agent for flow passage

The present invention is to provide a cell culture vessel comprising a copolymer which contains a recurring unit containing an organic group of the following formula (a) and a recurring unit containing an organic group of the following formula (b) being coated onto a surface thereof, a method for manufacturing the same and a method for manufacturing a cell aggregate using the same (wherein U, U, U, Uand U, and Anare as defined in the present specification and the claims). 

Cell culture vessel

The invention provides a ligand-bonded fiber in which a ligand having affinity for a cell membrane receptor is immobilized on a fiber precursor, and a cell culture substrate capable of repeating ex vivo amplification of a cell expressing a cell membrane receptor by using the ligand-bonded fiber.

Ligand-binding fiber and cell culture substrate using said fiber

The present invention provides a copolymer obtainable by polymerizing a monomer mixture which contains at least compounds of the following formulae (A), (B) and (C), wherein T, T, T, U, U, U, Uand U, Q, Qand Q, R, Rand R, Anand m are as defined in Specification and Claims, and so on. The copolymer of the present invention can be utilized as an ion complex material excellent in a function of inhibiting adhesion of a biological substance. 

The present invention provides a production method of a medium composition, including previously removing divalent metal cations contained in a polymer compound having an anionic functional group, preparing a dry powder of the polymer compound, and mixing the dry powder and the medium, and the like. The present invention also provides a purification method of a polymer compound having an anionic functional group, which includes mixing a suspension of the polymer compound having an anionic functional group in water and a cation exchanger that exchanges a divalent metal cation with a monovalent metal cation to give a polymer compound having an anionic functional group, which shows a reduced amount of contaminating divalent metal cations.

Method for producing culture medium composition

The present invention provides a culture method of cells and/or tissues including culturing cells and/or tissues in a suspended state by using a medium composition having an effect of preventing sedimentation of cells and/or tissues, which is afforded by substantially retaining the cells and/or tissues without substantially increasing the viscosity of the solution by nanofibers which have been added to the solution and uniformly dispersed in the liquid medium, and the like.

Culture medium composition

The present invention provides a method of isolating cells or tissues from a culture preparation of the cells or tissues in a medium composition which enables culture of the cells or tissues in suspension, which comprises at least one step selected from the group consisting of the following (A), (B) and (C):

Method for cell recovery

A composition for producing a fiber, containing (A) a polymer compound containing a unit structure represented by the formula (1) and a unit structure represented by the formula (2), (B) a crosslinking agent, (C) an acid compound, and (D) a solvent wherein each symbol in the formulas (1) and (2) is as described in the DESCRIPTION.

Active-ester-group-containing composition for producing fibers, and cell culture scaffold material using fibers produced from active-ester-group-containing composition

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Shiraoka, Japan

Taito Nishino