National Institute of Advanced Industrial Science and Technology

National Institute of Technology and Evaluation

National Research Institute of Brewing

Amano Enzyme Inc.

Taishin Kin

Kiyoshi Asai

Masayuki Machida

Keietsu Abe

Katsuya Gomi

Motoaki Sano

Naotake Ogasawara

Hideki Nagasaki

Akira Hosoyama

Osamu Akita

Satoru Kuhara

Shotaro Yamaguchi

Yasuji Koyama

Katsuhiko Kitamoto

Kotaro Ito

Kenichiro Matsushima

Atsuki Toumoto

Kensuke Yuuki

Manabu Arioka

Satoru Hisahara

Misao Sunagawa

Tasuku Nakajima

It is intended to provide koji mold-origin phospholipase Aand a DNA encoding it. Namely, phospholipase Acomprising the following protein (a) or (b): (a) a protein having an amino acid sequence represented by SEQ ID NO: 1 or 2; and (b) a protein having an amino acid sequence derived from an amino acid sequence represented by SEQ ID NO: 1 or 2 by a partial modification and serving as phospholipase A.

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Lysyl oxidase derived from filamentous fungi and a DNA encoding thereof are provided. Lysyl oxidase including a protein described in of the following (a) or (b): (a) a protein having an amino acid sequence set forth in SEQ ID NO: 2; or (b) a protein having an amino acid sequence obtained by modifying a part of the amino acid sequence set forth in SEQ ID NO: 2, and functioning as lysyl oxidase.

Fungus-origin lysyl oxidases

The purpose of the present invention is to isolate a gene encoding novel exo-, or endo-&#x3b2;--glucanase, to obtain a microorganism having an enhanced expression of said gene and to degrade &#x3b2;--glucan to its low molecular weight form by means of said enzymes. The present invention relates to a gene or DNA encoding novel enzymes having &#x3b2;--glucanase activity (exo-, or endo-&#x3b2;--glucanase), a recombinant expression vector comprising them, a microorganism having the recombinant expression vector, the novel enzymes having &#x3b2;--glucanase activity, and a method for the production of said enzymes.

Genes of cell wall-degrading enzymes derived from aspergillus, and method for the production the enzymes

A completely novel glutaminase is provided: (a) a protein consisting of an amino acid sequence represented by SEQ ID NO: 2, or (b) a protein consisting of an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO: 2 by deletion, substitution or addition of one or more amino acids, and possessing glutaminase activity. This protein is a novel glutaminase possessing excellent thermostability and the like.

Thermostable glutaminase and thermostable glutaminase gene

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Tokyo, Japan

Taishin Kin