Nihon Shokuhin Kako Co., Ltd.

Amano Enzyme Inc.

Yaizu Suisan Kagaku Kogyo Co., Ltd.

Yaizu Suisan Kagaku Kogyou Co., Ltd.

Taichi Usui

Masamichi Okada

Teruo Nakakuki

Atsuki Toumoto

Kazutaka Tsuruhami

Koichi Ogawa

Kanzo Sakata

Osamu Uejima

Kazuo Sakai

Shigeru Yamamoto

This invention relates to a novel microorganism-derived enzyme having an activity to cut disaccharide glycosides (particularly, &#x3b2;-primeveroside and/or analogous disaccharide glycosides) in disaccharide unit, a method for producing the enzyme, a gene which encodes the enzyme and use of the enzyme. Various components can be formed by the action of this enzyme upon disaccharide glycosides.

Diglycosidase isolated from microorganisms

Galactosyl maltooligosaccharide derivatives represented by formula (1): ##STR1## wherein at least one of X.sub.1 and X.sub.2 represents a galactosyl group and the other represents a hydrogen atom, R represents a hydrogen atom or a substituted or unsubstituted phenyl group, and n is an integer of from 2 to 5. The derivatives are prepared by reacting a sugar with a galactosyl group with a maltooligosaccharide represented by formula (2): ##STR2## R represents a hydrogen atom or a substituted or unsubstituted phenyl group, and n is an integer of from 2 to 5; in the presence of .alpha.-galactosidase or .beta.-galactosidase. The derivatives are used for measurement of .alpha.-amylase activity.

Galactosyl maltooligosaccharide derivatives

Highly purified derivatives of maltooligosaccharides can be produced at a high yield by reacting, in a mixture of a hydrophilic organic solvent and water, a mixture of maltooligosaccharides or a substance capable of being converted into the maltooligosaccharides upon reaction with an amylase, and an o-glucosyl derivative, with the amylase. The resulting derivatives of maltooligosaccharides are useful as a substrate for the determination of .alpha.-amylase activity in a humor, physiologically active substances, natural dieteic sweetenings, coloring agents and the like.

Process for the preparation of derivatives of maltooligosaccharides

Growing community of inventors

Shizuoka, Japan

Taichi Usui