Linden Technologies, Inc.

Hoffmann-La Roche Inc.

Shionogi Bioresearch Corporation

Tai-Nang Huang

Ming Shen

Li Chen

Jefferson Wright Tilley

Robert W Guthrie

Kenneth Gregory Hull

Achytharao Sidduri

Yih Huang

John Joseph Partridge

Weimin Liu

Achyutharao Sidduri

Percy Sarwood Manchand

Simon W Law

Steve Yik-Kai Tam

Haisun Liao

Guiqing Liang

Teresa Przewloka

Amy Anderson Deik

Shijie Zhang

Natalia Mamaeva

Peter S Belica

Caroline Ngaara Woodward

Shin-Yih Chen

This invention discloses dialysis devices, related housings, related membranes, related kits, and related methods.

Dialysis device

The invention features linker molecules that have at one terminus a amino-protecting group and at the other terminus a phosphorous activating group, typically phosphoramidite. The linker molecules can be used, for example, to produce amino-modified linkers that space an oligonucleotide from a solid support. The invention also features an amino-protected nucleotide that includes an activated phosphorous group such as a phosphoramidite for the production of a 5' amino-modified oligonucleotide. The invention further provides a phthalimido-modified support that can be used to synthesize a polynucleotide that includes an amino group at the 3&#x2032; terminus.

Protected linker compounds

Nucleotide preparations are transferred from a first substrate to a second substrate. One transfer method includes forming a patterned dry particulate deposition on a first substrate; positioning the first substrate in apposition to a second substrate; and transferring at least a portion of the dry deposition from the first substrate to the second substrate to produce a patterned dry deposition of the nucleotide on the second substrate. The method can be used to form an array of nucleic acids.

Transfer of arrayed chemical compositions

Disclosed is an insoluble support that can be used, for example, for producing replicates of sample nucleic acids. The support includes a plurality of attached oligonucleotides that include a prokaryotic promoter sequence and a target annealing sequence 3' of the promote. The proximal end of the promoter sequence is spaced from the insoluble support by a distance greater than 10 nm.

Nucleic acid amplification

Disclosed are compositions that include triboelectrically chargeable nucleotide particles of less than 50 &#x3bc;m diameter and carrier particles. In one example, a substrate is selectively patterned with the compositions, e.g., by transfer from a selectively charged surface. The compositions can be used to synthesize nucleic acid arrays.

Particulate compositions for chemical synthesis

N-alkanoylphenylalamine derivatives

Immobilized nucleotide primers of this invention include a modified nucleotide tethered to a support substrate through a linking group. In particular, the modified nucleotide is constructed such that the C-5&prime; end of the nucleotide is tetherable to the linking group and the protected C-3&prime; end is available for further controlled modification, e.g., addition of other nucleotides in specific sequences to the immobilized nucleotide primer. Additionally, the linking group is of sufficient length to allow the immobilized nucleotide primer to be used to synthesize and screen arrays of oligonucleotides, e.g., enzymatic C-3&prime; primer extension.

C-3&prime; protected monomeric nucleotides and synthesis of oligonucleotides on solid support

N-alkanoylphenylalanine derivatives

A cyanoguanidine compound of the following formula:

Cyanoguanidine compounds

N-aroylphenylalanine derivative VCAM-1 inhibitors

A method for the synthesis of 2',3'-dideoxycytidine comprising a novel procedure of bromoacetylating cytidine to yield novel intermediates which are subsequently reduced and hydrogenated by novel procedures to yield 2',3'-dideoxycytidine.

Intermediate compounds and an improved procedure for the synthesis of

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Lexington, MA, United States of America

Tai-Nang Huang