General Life Biotechnology Co., Ltd.

Development Center for Biotechnology

Tai-Guang Wu

Chein-Shyong Su

Liahng-Yirn Liu

Tong H Chang

Jiuan J Liu

Miao-Ling Hung

Tsai Yun Lee

Hung-Hsiu Ho

Ming-Yen Kuo

Wen-Jing Cheng

Hsueh-Fang Chen

Shi-Yuan Sheu

Chia-Wei Chang

Yi Chih Lei

Yu Fen Tsai

An electrochemical method and a test strip for detecting hemoglobin in a specimen are provided. The method includes the steps of providing the specimen with a reagent including a buffer solution, a surfactant and an electron mediator, tetrathiafulvalene, modified by cyclodextrin; detecting electric current produced by reaction of the hemoglobin and the electron mediator in the specimen under a potentiostatic condition; and calculating a concentration of the hemoglobin in the specimen according to the detected electric current.

Electrochemical method for detecting hemoglobin

An electrochemical biosensor formed by screen printing and method of fabricating such biosensor is disclosed in the present invention. The biosensor can quickly absorb a sample to be measured therein, effectively control volume of the sample fed and 'fill-and-position' the sample therein. The biosensor includes an electrode layer (electrode area) comprising two or three electrodes, which are a working electrode, a reference electrode and an auxiliary electrode (tri-electrode) on an insulating substrate. An active reaction layer containing reactant, reaction catalyst, mediator, wetting agent and surfactant is spread on the surface of the electrode layer. A sample inflow area is formed above the electrode area by adding an upper cover on top of a middle insulating layer with a U-shaped opening formed therein. Sample solution with a minute amount about 0.8 to 1 &#x3bc;l can be rapidly introduced into the electrode area and the active reaction layer via the inflow area by siphon or capillary, where the ingredient of the sample can be analysed by measuring reaction between the sample, reaction catalyst and mediator in the reaction layer using electrochemical potentiometric or amperometric method. An upwardly extended closed space formed within the upper cover above the electrode area adjacent to the front of conductive wires can be effectively used to control sample volume and 'fill-and-position' the sample.

Electrochemical biosensor by screen printing and method of fabricating same

Interference-eliminating membranes, test strips, kits and methods for use in uric acid assay

Growing community of inventors

Taipei, Taiwan

Tai-Guang Wu