Samsung Electronics Co., Ltd.

Tae-joon Kwon

Kyu-sang Lee

Ji-young Oh

A method of designing primer and probe sets for identification of target sequences by amplification and hybridization is provided. Also, primer and probe sets designed by the method, a kit comprising the sets, a computer readable medium recorded thereon a program to execute the method, and a method of identifying target sequences using the sets are provided. Accordingly, primer and probe sets capable of identifying rapidly and accurately a number of target sequences can be readily designed.

Method of designing primer and probe sets, primer and probe set designed by the method, kit comprising the sets, computer readable medium recorded thereon program to execute the method, and method of identifying target sequence using the sets

A method of designing a probe set for identifying a target sequence from a plurality of target sequence groups by a hybridization reaction is provided. The method includes: (a) selecting a first target sequence group including a plurality of target sequences; (b) selecting oligonucleotides specifically binding to the target sequences from the first target sequence group as a probe; (c) selecting one or more target sequences having no specifically binding probe from the first target sequence group as a second target sequence group; (d) selecting oligonucleotides specifically binding to the target sequences from the second target sequence group as a probe, wherein the operations (c) and (d) are repeated until a target sequence having no specifically binding probe is absent in the second target sequence group.

Method of designing probe set, microarray having substrate on which probe designed by the method is immobilized, and computer readable medium on which program for executing the method is recorded

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Gyeonggi-do, South Korea

Tae-joon Kwon