Advanced Protein Technologies Corporation

Samyan Genex Corporation

Tae-Hoon Cho

Yeon-Sung Park

Moon-Suk Kim

Jong-Won Yoon

Woo-Jong Lee

Heung-Bok Park

Jeong-Min Kim

Bu-Soo Park

Bok-Luel Lee

Ji-Won Park

Jeong-Mi Yeo

Hyun-Sic Kim

Chang-Hun Joo

Seung-Hwan Song

Hong-Lak Kim

Joong-Hyuck Auh

The present invention relates to a process of separation a protein of interest comprising expressing the protein of interest fused with a fusion partner, selectively adsorbing on matrix, and performing efficient cleavage reaction on the fusion protein adsorbed on matrix or separated from the matrix, and recovering the protein of interest. Also, the present invention provides a fusion protein comprising a protein of interest and a fusion partner, wherein the fusion partner comprises an amino acid sequence which can be cleaved by ubiquitin cleavage enzyme at its C-terminus, and which has a difference in isoelectric point of 1 or more from the protein of interest. According to the present invention, the proteins of interest can be purified in high yield and purity. In addition, complicated separating processes required in the production of recombinant proteins can be eliminated so as to cut down the production cost.

Process for preparation of polypeptides of interest from fusion polypeptides

The present invention relates to a composition for selectively detecting an extremely small amount of peptidoglycan in sample, a preparation method of the composition, and a detection kit for peptidoglycan. It is possible to quantify a small amount of peptidoglycan contained in human blood, tissue, body fluid, water or food, and to diagnose an infection of microorganism with peptidoglycan as a component of cell wall using the composition and the detection kit. In addition, the composition can be applied for a diagnosis reagent of detecting an infection of Gram-positive bacteria in animal or human being in advance, and thus, can be used for the prevention or treatment of food poisonings and bacterial sepsis.

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Growing community of inventors

Suwon, South Korea

Tae-Hoon Cho