Toyo Boseki Kabushiki Kaisha

Nikko Bio Technica Co., Ltd.

Shionogi Company Limited

Kyoto University

Ezaki Glico Co., Ltd.

Toyobo Co., Ltd.

Tadayuki Imanaka

Yoshihisa Kawamura

Masao Kitabayashi

Masahiro Takagi

Bunsei Kawakami

Masaaki Morikawa

Yoshiaki Nishiya

Hiroaki Inoue

Hideyuki Komatsubara

Taku Arakawa

Shoji Sakurai

Toshihiro Kuroita

Yoshikazu Ishida

Takeshi Takaha

Shigetaka Okada

Hiroshi Aiba

Hiroki Takata

Michiyo Yanase

Kazutoshi Fujii

Yoshinobu Terada

Masanori Oka

Hiroyasu Nakamura

Hideki Kamimura

Shuichi Aiba

Haruyuki Atomi

Toshihiro Tsuruga Inst Of Biotechnology Kuroita

Masao Tsuruga Inst Of Biotechnology Kitabayashi

Yoshikazu Tsuruga Inst Of Biotechnology Ishida

Yoshiaki Tsuruga Inst Of Biotechnology Nishiya

Hideyuki Tsuruga Inst Of Biotech Komatsubara

Yoshihisa Tsuruga Inst Of Biotechn Kawamura

Bunsei Tsuruga Inst Of Biotechnology Kawakami

The invention provides a glucose dehydrogenase that is an extremely stable enzyme having a thermostability of 80&#xb0; C. or more, and that does not substantially act upon saccharides other than glucose (e.g., having a reactivity of less than 3% with respect to maltose, galactose, and xylose). The invention also provides a method for producing such an enzyme, and a composition for quantifying glucose using such an enzyme.

Glucose dehydrogenase

An object of the present invention is to provide a thermostable DNA polymerase with enhanced amplification efficiency and/or improved fidelity in polymerase chain reaction (PCR), and provide a process for production thereof. More specifically, the present invention provides thermostable DNA polymerase wherein in the DXEXXXH sequence (D: aspartic acid, E: glutamic acid, H: histidine, X, X, Xand X: any amino acid) consisting of DXE sequence within the EXO I region and a four amino acid length peptide adjacent to said glutamic acid(E) of thermostable DNA polymerase having 3'-5&#x2032; exonuclease activity, histidine(H) has been replaced by another amino acid.

Modified thermostable DNA polymerase

The invention provides methods, kits, and compositions for enhancing synthesis of DNA involving a carboxylate ion-supplying substance that is effective in promoting DNA synthesis in enzymatic DNA synthesis reactions. The invention further provides a thermostable DNA polymerase-related factor derived fromspecies, which has an activity to promote the DNA synthesis activity of DNA polymerase or which binds to DNA polymerase.

Compositions for enhancing DNA synthesis, DNA polymerase-related factors and utilization thereof

Glucan with a degree of polymerization of 50 or more includes an inner branched cyclic structure portion and an outer branched structure portion, and methods for producing the same.

Glucan having cyclic structure and method for producing the same

A nucleic acid amplifying enzyme having a short reaction time and high fidelity is provided. The enzyme of this invention is a thermostable DNA polymerase having a nucleic acid extension rate of at least 30 bases per second and a 3&prime;-5&prime; exonuclease activity. Also provided are a method and kit for amplifying nucleic acid.

Kit for amplifying nucleic acid

DNA encoding a thermostable DNA polymerase

A nucleic acid amplifying enzyme having a short reaction time and high fidelity is provided. The enzyme of this invention is a thermostable DNA polymerase having a nucleic acid extension rate of at least 30 bases per second and a 3'-5' exonuclease activity. Also provided are a method and kit for amplifying nucleic acid.

Methods of amplification using a thermostable DNA polymerase from the

Thermostable DNA polymerase from a hyperthermophilic archaeon strain KOD1

A modified thermostable DNA polymerase having 5% or less of the 3'-5' exonuclease activity of the enzyme before modification and a DNA polymerase composition for amplifying nucleic acid, which comprises the modified thermostable DNA polymerase having 0 to 5% of the 3'-5' exonuclease activity of the enzyme before modification and an unmodified thermostable DNA polymerase having 3'-5' exonuclease activity or a modified thermostable DNA polymerase having 100 to 6% of the 3'-5' exonuclease activity of a thermostable DNA polymerase before modification; a method for amplifying nucleic acid by use of said modified thermostable polymerase or said DNA polymerase composition; and a reagent therefor.

Modified thermostable DNA polymerase derived from pyrococcus sp. KOD and

A novel biosurfactant having a high surface activity, as well as a microorganism producing the surfactant is disclosed. The biosurfactant according to the present invention is represented by the formula [I]. ##STR1## The present invention also provides Arthrobacter sp. No. 38 (FERM BP-4435) which produces the biosurfactant of the formula [I].

Biosurfactant cyclopeptide compound produced by culturing a specific

Disclosed is a method of super precision devices such as semiconductor devices. In the method of the present invention, the devices are washed with an aqueous solution containing a purified proteolytic enzyme, poly(oligo)saccharide-decomposing enzyme, or a lipid or oil-decomposing enzyme.

Method of washing super precision devices, semiconductors, with enzymes

A novel strain belonging to Bacillus stearothermophilus. The strain of the present invention has a protoplast-forming ratio of not less than 99% and has a ration of regeneration of cell wall of not less than 50%, and is transformable with a plasmid.

Bacillus stearothermophilus strain having a high protoplast forming ratio

A process for transformation of a microorganism by introduction of a plasmid therein, the microorganism being thermophilic and gram-positive or gram-variable, and vector plasmids used therefor.

Transforming Bacillus stearothermophilus with plasmid vector pTB 19

Growing community of inventors

Suita, Japan

Tadayuki Imanaka