Suntory Holdings Limited

Tadayoshi Hirai

Kazunari Iwaki

Katsuro Miyagawa

Eiichiro Ono

Saori Takeyama

Kentaro Ochiai

Misa Ochiai

Naoko Okitsu

Koji Nagao

Soichiro Urai

Takehiro Watanabe

Kohki Fujikawa

A method of screening for a stevia plant with high rebaudioside M content ratio, that includes detecting from a test stevia plant the presence and/or absence of at least one of (1) and (2), and at least one of (3) to (7): (1) homozygous for the allele wherein position 290 of SEQ ID NO: 1 is T; (2) homozygous for the allele wherein position 33 of SEQ ID NO: 2 is A; (3) homozygous for the allele wherein position 44 of SEQ ID NO: 3 is T; (4) homozygous for the allele wherein position 40 of SEQ ID NO: 4 is T; (5) homozygous for the allele wherein position 48 of SEQ ID NO: 5 is C; (6) homozygous for the allele wherein positions 55-72 of SEQ ID NO: 6 are deleted; (7) homozygous for the allele wherein position 50 of SEQ ID NO: 7 is A.

Stevia plant having high rebaudioside M content ratio and screening method for same

The present invention provides aplant having a low ability to form pollens as compared with the wild typespecies. The present invention also provides a method of producing such aplant having a low ability to form pollens, and an extract or a steviol glycoside purified product obtainable from such a plant.

Stevia plant having less ability to form pollens

A method of screening for a stevia plant with high steviol glycoside content, that includes detecting from the genome of a test stevia plant the presence and/or the absence of the following genetic feature: (1) homozygous for the allele wherein the base at the position corresponding to position 290 of SEQ ID NO: 1 is T, and the presence and/or the absence of the following genetic feature: (2) homozygous or heterozygous for the allele wherein the base at the position corresponding to position 40 of SEQ ID NO: 2 is A.

High steviol glycoside-containing stevia plant and method for screening same

The present invention provides a high rebaudioside M content non-genetically modifiedplant comprising rebaudioside M at higher content as compared with the wild typespecies. The present invention also provides a method of producing such a high rebaudioside M content non-genetically modifiedplant, and a dry leaf obtainable from such a plant.

Rebaudioside M-rich stevia plant

The present invention provides aplant having a low ability to form flower buds as compared with the wild typespecies. The present invention also provides a method of producing such aplant having a low ability to form flower buds, and an extract or a steviol glycoside purified product obtainable from such a plant.

Stevia plant having less ability to form flower buds

The purpose of the present invention is to: determine the structure of a novel steviol glycoside which is detected from species containing a large amount of Reb. C (also referred to as dulcoside B), and a trace amount of which is capable of influencing the quality of taste; and understand the taste characteristics of said steviol glycoside. The present invention provides a compound represented by formula (1) or a salt thereof, or a hydrate thereof.

Steviol glycoside and production method therefor, and sweetener composition containing same

The present invention provides a high sweet contentplant having a variation at a portion corresponding to SEQ ID NO: 1, a method of producing or screening for the same, etc.

High-sweetening-content stevia plant and method for screening same

The purpose of the present invention is to provide a steviol glycoside hexose transferase, and a method for producing a steviol glycoside that contains glucose and/or rhamnose using said enzyme. The present invention provides a steviol glycoside hexose transferase, and a method for producing a steviol glycoside that contains glucose and/or rhamnose using said enzyme. The present invention also provides a transformant into which a steviol glycoside hexose transferase gene has been introduced, and a method for preparing said transformant.

Steviol glycoside hexose transferase and gene coding for same

The purpose of the present invention is to provide a protein, said protein having an activity of synthesizing rhamnose from glucose, and a polynucleotide encoding the same. Provided are a rhamnose synthase derived from stevia and a method for producing rhamnose from glucose using a gene. Also provided is a method for producing a steviol glycoside using the rhamnose synthase derived from stevia.

Rhamnose synthase derived from stevia and gene

An objective of the present invention is to provide aplant having a high content of rebaudioside C. The present invention provides a high rebaudioside C-content type, non genetically modifiedplant that has a higher content of rebaudioside C than wild-typevarieties, more specifically at least 20% higher content. The present invention further provides: a method for producing such a high rebaudioside C-content type, non genetically modifiedplant; and dried leaves obtained from such a plant.

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Kyoto, Japan

Tadayoshi Hirai