Kao Corporation

Tadashi Yoshimatsu

Susumu Ito

Shuji Kawai

Tohru Kobayashi

Jun Hitomi

Mikio Takaiwa

Shigehito Adachi

Yoshihiro Hakamada

Yoko Watanabe

Katsuya Ozaki

Shitsuw Shikata

The present invention relates to a novel enzyme called alkaline proteinase K-16 that is characterized as having an optimum pH in an alkaline range, and stability in the presence of various surface active agents, such as sodium linear alkylbenzene sulfonate, sodium polyoxyethylene alkyl sulfate, sodium dodecyl sulfate, sodium .alpha.-olefin sulfonate, sodium alkyl sulfonate, and .alpha.-sulfo-fatty acid ester. The present invention further provides for the microorganism Bacillus sp. Ferm BP-3376, which is capable of producing the novel alkaline proteinase, and a process for producing the novel alkaline proteinase. Alkaline-proteinase K-16 of the present invention exhibits an excellent action against insoluble proteins and maintains sufficient activity over a wide temperature range and in the presence of surface active agents thus providing a use as an enzyme for detergents.

Bacillus SP. ferm BP-3376 and a method for its use to produce an

The present invention relates to a novel enzyme called alkaline proteinase K-16 that is characterized as having an optimum pH in an alkaline range, and stability in the presence of various surface active agents, such as sodium linear alkylbenzene sulfonate, sodium polyoxyethylene alkyl sulfate, sodium dodecyl sulfate, sodium .alpha.-olefin sulfonate, sodium alkyl sulfonate, and .alpha.-sulfo-fatty acid ester. The present invention further provides for a microorganism producing the novel alkaline proteinase, and a process for producing the novel alkaline proteinase. Alkaline proteinase K-16 of the present invention exhibits an excellent action against insoluble proteins and maintains sufficient activity over a wide temperature range and in the presence of surface active agents thus providing a use as an enzyme for detergents.

Alkaline proteinase isolated from bacillus sp.

A novel alkaline cellulase is disclosed. The alkaline cellulase is produced by Bacillus sp KSM-635 (FERM BP-1485) and possesses: (1) C.sub.x enzyme activity to act on carboxymethyl cellulose and weak C.sub.1 enzyme .beta.-glucosidase activities, (2) substrate specificity to act on carboxymethyl cellulose, cellulose cotton, Avicel, filter paper, cellobiose, and p-nitrophenyl cellobioside, (3) a working pH range of 4-12 and an optimum pH range of 9-10, (4) stable pH range of 4.5-10.5 when allowed to stand at 40.degree. C. for 10 minutes, and (5) working temperature range of 10.degree.-65.degree. C. and optimum temperature range at around 40.degree. C. Its activity is not inhibited by chelating agents nor by surface active agents. It is particularly effective as an additive for laundry detergent compositions.

Alkaline cellulase and process for producing the same

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Utsunomiya, Japan

Tadashi Yoshimatsu