Other

Tokyo University of Agriculture and Technology

Tdk Corporation

Hitachi Chemical Company, Ltd.

Yokogawa Electric Corporation

Pentel Kabushiki Kaisha

Kabushiki Kaisya Advance Kaihatsu Kenkyujo

Kuraray Co., Ltd.

The City University of New York

Toray Silicone Company Limited

Asahi Denka Kogyo K.k.

Matsunaga, Tadashi

Tadashi Matsunaga

Masahito Hosokawa

Hitoshi Wake

Tomoko Yoshino

Hideki Kobayashi

Yoshihito Kikuhara

Masafumi Kanetomo

Saya Satou

Haruko Takeyama

Kazuhisa Fukushima

Kiyoshi Hishinuma

Mayumi Ono

Hironori Umetsu

Tsuyoshi Tanaka

Kenryo Namba

Hisashige Kanbara

Takanori Kitamura

Hiroshi Matsui

Nobuyuki Murakami

Tomoyuki Taguchi

Shinji Kamiya

Hiromichi Takahashi

Tsuruo Nakayama

Brandon Yoza

Ryo Negishi

Noriyuki Nakamura

Kin-ichi Ozawa

Seita Nakamura

Toshihiro Takimoto

Hideo Kadoi

Atsushi Arakaki

A cell isolation method includes: a cell trapping step of allowing a test liquid to pass through a cell trapping filter which has a plurality of through-holes in the thickness direction, thereby trapping isolation target cells contained in the test liquid on one surface of the cell trapping filter; a gel embedding step of introducing a stimulus-responsive hydrogel onto the one surface of the cell trapping filter on which the cells have been trapped in the cell trapping step, thereby embedding the cells in the stimulus-responsive hydrogel; a gel hardening step of applying a stimulus to the stimulus-responsive hydrogel in which the cells are embedded, thereby hardening the stimulus-responsive hydrogel; and a detachment step of detaching the stimulus-responsive hydrogel that was hardened in the gel hardening step from the cell trapping filter.

Cell isolation method and cell trapping filter

Component for a cell-collecting cartridge

The instant invention is directed to a microfluidic device which separates and captures with high efficiency a large amount of cells in a sample at one-cell level without damaging the cells by utilizing a microfabrication technology.

Microfluidic device for trapping single cell

A magnetic nanotube includes bacterial magnetic nanocrystals contacted onto a nanotube which absorbs the nanocrystals. The nanocrystals are contacted on at least one surface of the nanotube. A method of fabricating a magnetic nanotube includes synthesizing the bacterial magnetic nanocrystals, which have an outer layer of proteins. A nanotube provided is capable of absorbing the nanocrystals and contacting the nanotube with the nanocrystals. The nanotube is preferably a peptide bolaamphiphile. A nanotube solution and a nanocrystal solution including a buffer and a concentration of nanocrystals are mixed. The concentration of nanocrystals is optimized, resulting in a nanocrystal to nanotube ratio for which bacterial magnetic nanocrystals are immobilized on at least one surface of the nanotubes. The ratio controls whether the nanocrystals bind only to the interior or to the exterior surfaces of the nanotubes. Uses include cell manipulation and separation, biological assay, enzyme recovery, and biosensors.

Magnetic nanotubes

The present invention relates to a method of extracting nucleic acid or protein, in which multi-layer dendrimers are formed on the surface of fine particles, amino radicals for capturing nucleic acid or protein are formed on the surface of these dendrimers, and nucleic acid or protein is extracted using these amino radicals. The present invention can greatly and easily increase the recovery ratio of nucleic acid or protein.

Method of extracting nucleic acid or protein using dendrimers and dendrimer-compositional substances

The present invention provides a dendrimer-based biochip, wherein a flow channel through which a solution containing biopolymer molecules is flowed is formed in the substrate of the biochip, a plurality of dendrimer molecules one end of each of which is bound to the walls of the flow channel are formed thereon, and probe biopolymer or antibody molecules are bound to the tips of the dendrimer molecules so that, if the probe biopolymer molecules are bound, then target biopolymer molecules can be captured by means of a complementary combination and, if the antibody molecules are bound, then protein can be extracted by means of antigen-antibody reaction, whereby biopolymers can be retrieved in a highly efficient manner.

Dendrimer-based DNA extraction methods and biochips

An electrochemical stain prevention apparatus of a submerged structure comprising a submerged structure of which at least the stain prevention surface is formed of a conductive film that does not generate chlorine even by applying a potential of 5 V vs. SCE or less, a counter electrode located so as not to contact with the submerged structure, and a power supply unit for passing a direct current through the submerged structure having the conductive film formed thereon and the counter electrode. Aquatic organisms adhered to the surface of the conductive film can effectively be controlled by applying a potential of from 0.1 to 5 V vs. SCE to the submerged structure of such a stain prevention apparatus without generating chlorine. A potential applied to the conductive film of the submerged structure can be controlled with good accuracy by disposing a reference electrode between the submerged structure and the counter electrode. As the conductive film formed on the substrate of the submerged structure, a sprayed coating film made of a metal nitride can preferably be used.

Electrochemical stain prevention apparatus of submerged structure and process for producing submerged structure used in this apparatus

A fusion DNA sequence, which is obtained by fusing a gene coding for another useful protein to a fragment of a magA gene coding for a protein bound to an organic membrane for covering magnetic particles produced in cells of a magnetic bacterium AMB-1, is expressed in the magnetic bacterium to obtain the protein in a state of being bound to the magnetic particles. According to the present invention, useful proteins such as enzymes and antibodies can be stably obtained in a state of being bound to the organic membrane of the magnetic particles only by cultivating a transformed magnetic bacterium, and separating the magnetic particles produced in cells, without any necessity to perform a treatment such as immobilization. The functional protein immobilized on the magnetic particles can be magnetically controlled. Thus the function can be efficiently exhibited at a desired topical position. Magnetic particles to which a desired protein is bound can be semipermanently produced only by maintaining and cultivating an identical bacterial strain. Since the protein is produced on the magnetic particles, an objective protein can be magnetically separated and recovered in a short period of time. Thus it is possible to perform efficient separation and purification.

Protein-bound magnetic particles and process of producing the same

Fine magnetic particles containing useful proteins bound thereto,

Fusion protein-bound magnetic particles for recombinant production and

Processes for detecting a sensitized leukocyte or an antigen in a liquid sample are described. One of the processes contains the steps of: adding a known antigen to the liquid sample, or the liquid sample to a known sensitized leukocyte; applying an electric potential between a working electrode immersed in a mixture of the known antigen or the known leukocytes and the liquid sample, and a counter electrode, to generate an electric current therein; and measuring an amount of the electric current generated. Further, another process contains the steps of: adding a known antigen to said liquid sample, or the liquid sample to a known sensitized leukocyte; and measuring an amount of serotonin or histamine released thereby. Still further, apparatuses for carrying out the above processes are also described.

Process and apparatus for detecting sensitized leukocyte or antigen

A process for producing L-phenylalanine at high rates of formation and in good yields is provided which comprises allowing a microorganism which belongs to the genus Nocardia and is capable of producing L-phenyl-alanine from phenylpyruvic acid or a salt thereof and an amino group donor to act upon an aqueous solution containing phenylpyruvic acid or a salt thereof and an amino group donor selected from the group consisting of ammonium salts and ammonia in a hydrogen gas atmosphere.

Process for producing L-phenylalanine

A method of plant tissue culture which comprises culturing a tissue or an organ of a plant, a part of the same or cultured cells in a medium containing a culture filtrate and/or an extract of a photosynthetic procaryotic microorganism. Such a culturing method effectively proliferates the plant tissues and cultured cells, and also promotes the formation of adventive embryos, regeneration of the plant body and production of useful substances formed by that plant. Strains of cyanobacteria or photosynthetic bacteria are preferably used as the photosynthetic procaryotic microorganism.

Method of plant tissue culture

Growing community of inventors

Fuchu, Japan

Tadashi Matsunaga