Mochida Pharmaceutical Co., Ltd.

Tadakazu Yamauchi

Masahiro Nobuhara

Ei Mochida

Hiroshi Sato

Toshinori Kanamori

Toshio Izako

Hideyuki Terasawa

Keiko Kuzuya

Process for specific binding assay for measuring the amount of analyte in a liquid test sample

Improvement in assays utilizing at least hydrogen peroxide for one analysis reagent is provided. The assay of the present invention employs a stable hydrogen peroxide adduct in dry state which has no adverse effects on the assay, and which has a high hydrogen peroxide-retaining ability. In the assay, an aqueous solution is added to an adduct in dry state of (a) at least one member selected from the group consisting of a carboxylic acid and a salt thereof, phosphoric acid and a salt thereof, and a sulfonic acid and a salt thereof, and (b) hydrogen peroxide to generate peroxide, and the thus generated peroxide is used for the analysis reagent.

Assay utilizing hydrogen peroxide adduct

Medical measurement apparatus

The present invention provides a general purpose specific binding assay method which has the advantages of highly accurate and quick measurements which exclude the effects of various factors that decrease reliability of the measured values, such as non-specific reactants in test samples, assay conditions and inactivation and the like changes in the activity of reagents. The present invention is further drawn to a specific binding assay device suitable for the practice thereof. The binding assay of the present invention is achieved by allowing a signal substance generator which takes part in a specific binding reaction and generates a signal substance, together with a liquid sample, to flow through a predetermined channel in a predetermined direction, thereby effecting generation of the specific binding reaction of a substance to be assayed to form a distribution of the signal substance generator in the channel in response to the concentration of the substance to be assayed, allowing the signal substance generator distributed in the channel to generate the signal substance, detecting the generated signal substance by a plurality of detection means arranged at different positions in the liquid flow direction, and arithmetically processing the plural detection results to minimize influence of other factors than the concentration of the substance to be assayed upon the assay result.

Method and device for specific binding assay

An enzyme electrode, specific binding or the like electrochemical assay method capable of performing always stably high detection sensitivity (responsibility) and reappearance even in the case of blood, urine and the like samples that contain interfering substances and also of applying suitably to disposable use, and a novel p-phenylenediamine compound which is used in the assay method. Particularly, an electrochemical assay method in which a substance in a liquid sample is assayed using at least one oxidoreductase, wherein an oxidoreductase, an electron mediator and an electrode which performs electron transfer with the mediator are arranged in the assay system, and a compound of the following formula [I] or a salt thereof is used as the mediator which is highly soluble in water, dried (by freeze-, vacuum- or air-drying) easily and stable under dry condition, shows a high electron transfer rate with enzymes and is almost free from the influence of interfering substances in blood, urine and the like samples: ##STR1## wherein R.sup.1 to R.sup.4 may be the same or different from one another and each means a hydrogen, a straight- or branched-chain alkyl group having 1 to 4 carbon atoms, optionally having a substituent, providing that at least one of R.sup.1 to R.sup.4 has one or more groups selected from hydroxyl, mercapto, carboxyl, phosphonooxy and sulfo.

Electrochemical assay method with novel p-phenylenediamine compound

A specific binding assay process which is useful for quick qualitative or quantitative measurement and which can be used for various purposes, and a specific binding assay device suitable for the practice of the process,

Electrochemical immunochromatographic assay

A reagent for use in an immunoassay for measuring haptens, antigens or antibodies by means of a competitive binding method, which comprises a combination of an antibody and a labelled hapten or a labelled antigen or a combination of a hapten or an antigen and a labelled antibody, wherein the antibody and the labelled hapten or the labelled antigen in one combination or the hapten or the antigen and the labelled antibody in another combination are capable of undergoing reversible binding, and a device for use in an immunoassay wherein the reagent of the present invention is included in a single container. An immunoassay can be performed in a short time by the use of the immunoassay device of the present invention.

Reagent for immunoassay, and device using the same

A reagent for use in an immunoassay for measuring haptens, antigens or antibodies by means of a competitive binding method, which comprises a combination of an antibody and a labelled hapten or a labelled antigen or a combination of a hapten or an antigen and a labelled antibody, wherein the antibody and the labelled hapten or the labelled antigen in one combination or the hapten or the antigen and the labelled antibody in another combination are capable of undergoing reversible binding, and a device for use in an immunoassay wherein the reagent of the present invention is included in a single container. This immunoassay can be performed in a short time by the use of the immunoassay device of the present invention.

Growing community of inventors

Saitama, Japan

Tadakazu Yamauchi