Fujifilm Corporation

Tadahiro Matsuno

Noriyuki Kasagi

Toshihiro Mori

Toshihito Kimura

Hideyuki Karaki

Kentaro Nakamura

Junichi Katada

Akihiro Komatsu

Nobuhiko Fujiwara

Kouta Katou

An assay chip includes fluidic-channel member composed of a light-transmissive lower member and an upper member, forming a fluidic-channel therebetween, and a cover member fitted with the fluidic-channel member from the upper-member-side thereof. An inlet for injecting a sample solution into the fluidic-channel and a suction opening for sucking, from the downstream side, the injected sample solution, both communicating with the fluidic-channel, are formed on the upper surface of the upper member. A pot for carrying out predetermined pre-processing on the sample solution, a pot for first-reaction processing to bind a photoresponsive labeling substance to an analyte in the sample solution, an inlet insertion-hole for inserting the inlet, and a suction-opening insertion-hole for inserting the suction opening are linearly arranged on the upper surface of the cover member.

Assay chip

Provided are a base plate and a method for thyroxine immunoassay, which are capable of decreasing the signal change rate caused by temperature change in a high-sensitivity immunoassay method for thyroxine. A base plate for thyroxine immunoassay which has a conjugate of thyroxine or a derivative thereof and albumin immobilized thereon, and in which the ratio of the molecule number of thyroxine or a derivative thereof to the molecule number of albumin in the immobilized conjugate, is disclosed.

Thyroxine immunoassay using fluorescent particles

It is an object of the present invention to provide a method for producing a conjugate of thyroxine and albumin with higher purity. The present invention provides a method for producing a conjugate of thyroxine and albumin which comprises: step (a) of converting a carboxyl group in thyroxine having a carboxyl group to be linked to albumin into an active ester and allowing the thyroxine to react with albumin, so as to prepare a conjugate of thyroxine and albumin; and step (b) of purifying the conjugate with the use of an acidic mixed aqueous solvent in which the thyroxine having a carboxyl group to be linked to albumin is dissolved but albumin is not precipitated.

Method for producing conjugate of thyroxine and albumin

It is an object of the present invention to provide an immunoassay method which is capable of simultaneous quantification of a plurality of test substances under a same analysis condition, through adjustment of the measurement sensitivity/range by simply varying the size of particles for use as labels, without changing the spectra of these particles. The present invention provides an immunoassay method which comprises: simultaneously or step-by step developing respective of the plurality of the test substances and respective of labeling substance particles labeled with first binding substance against the respective of the plurality of the test substances on an insoluble carrier; capturing the test substances and the labeling substance particles in reaction portions on the insoluble carrier that has been immobilized with the respective of second binding substances against the respective of the plurality of the test substances at different positions; and measuring optical characteristic of the labeling substance particles, so as to simultaneously detect the plurality of the test substances, wherein the plurality of the test substances having different detection concentration ranges are simultaneously detected with use of the labeling substance particles which are different in the particle size corresponding to the test substances.

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Ashigarakami-gun, Japan

Tadahiro Matsuno