Keygene N.v.

Taco Peter Jesse

Michael Josephus Theresia Van Eijk

Adrianus Johannes Van Tunen

The present invention relates to a method for identifying the source of an amplicon, comprising: providing a plurality of pools of amplicons from different sources, wherein the amplicons from different sources are present in more than one pool, and wherein the amplicons in each pool are tagged with a unique pool-specific identifier; sequencing at least part of the amplicons that comprise the pool-specific identifiers; and assigning one or more of the amplicons to corresponding pools and/or sources using the pool-specific identifiers.

Method for identifying the source of an amplicon

The present invention relates to a high throughput method for the identification and detection of molecular markers wherein restriction fragments are generated and suitable adaptors comprising (sample-specific) identifiers are ligated. The adapter-ligated restriction fragments may be selectively amplified with adaptor compatible primers carrying selective nucleotides at their 3' end. The amplified adapter-ligated restriction fragments are, at least partly, sequenced using high throughput sequencing methods and the sequence parts of the restriction fragments together with the sample-specific identifiers serve as molecular markers.

Method for genome sequencing using a sequence-based physical map

Method for the identification of the clonal source of a restriction fragment

The invention pertains to a method for the integration of physical and genetic maps and markers. The method is based on the use of AFLP fingerprinting with primers of varying selectivity on a library of artificial chromosomes such as a BAC library. The fingerprinting is performed on the individual BACs and on the pools of BACs. Subsequent alignment generates a contig and provides the integration of physical and genetic markers resulting in the integration of the physical and genetic map.

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Wageningen, Netherlands

Taco Peter Jesse