Pneumatic Scale Corporation

Other

T David Marro

Stephen B Kessler

Daniel S Sutton

S Kessler

An apparatus for separating cell suspension material into centrate and concentrate, includes a single use structure () releasably positioned in a cavity in a solid wall rotatable centrifuge bowl (). The bowl and portions of single use structure rotate about an axis (). A stationary inlet feed tube (), a centrate discharge tube () and a concentrate discharge tube () extend along the axis of the rotating single use structure. A centrate centripetal pump () is in fluid connection with the centrate discharge tube. A concentrate centripetal pump () is in fluid connection with the concentrate discharge tube. A controller () operates responsive to sensors () in respective centrate and concentrate discharge lines (), to control flow rates of a concentrate pump () and a centrate pump () to produce output flows of cell concentrate and generally cell free centrate.

Centrifuge system for separating cells in suspension

An apparatus for separating cell suspension material into centrate and concentrate, includes a single use structure () releasably positioned in a cavity in a solid wall rotatable centrifuge bowl (). The bowl and portions of single use structure rotate about an axis (). A stationary inlet feed tube (), a centrate discharge tube () and a concentrate discharge tube () extend along the axis of the rotating single use structure. A centrate centripetal pump () is in fluid connection with the centrate discharge tube. A concentrate centripetal pump () is in fluid connection with the concentrate discharge tube. At least one concentrate channel () and a concentrate centripetal pump chamber () have configurations in the structure that facilitate the flow of cell concentrate.

A method and apparatus for separating cell suspension into centrate and concentrate includes a solid wall rotatable centrifuge bowl (). A single use rotatable core () is positioned in the bowl and bounds a cavity () which serves as a separation chamber. The bowl rotates about an axis (). An inlet tube () and an outlet tube () extend along the axis in the bowl cavity. The centripetal pump () in the cavity is in connection with the outlet tube. The centrate discharge pump () is in connection with the outlet tube. A pressure damping reservoir () is fluidly connected between the outlet tube and the discharge pump. At least one control circuit () is operative to control the apparatus to maintain a positive pressure above atmospheric pressure within the cavity, so as to maintain the cell suspension away from at least one seal () that bounds the interior of the cavity.

Centrifuge system including a control circuit that controls positive back pressure within the centrifuge core

A centrifuge may include a bowl operative to rotate with respect to a stationary portion. The centrifuge may include at least one vibration sensor operative to generate vibration data representative of vibrational movement of portions of the centrifuge. The processor may monitor the vibration data as the bowl is being filled with a fluid. The processor may cause a drive device to increase the rotational speed of the bowl responsive to determining from the vibration data that the bowl has becoming substantially filled with a fluid.

Centrifuge system and method that determines fill status through vibration sensing

A method and apparatus for cell harvest of production scale quantities of cell cultures using single use components comprising a flexible membrane mounted on a rigid frame and is supported within a multiple use rigid centrifuge bowl, such single use components including a core with an increased diameter and an internal truncated cone shape in order to permit the system to maintain a sufficiently high angular velocity to create a settling velocity suited to efficiently processing highly concentrated cell culture streams. Features which minimize feed turbidity, and others which permit the continuous or semi-continuous discharge of cell concentrate, increase the overall production rate over the rate which can be achieved using current intermittent processing methods for large cell culture volumes. Injection of a diluent during the cell concentrate removal process permits more complete removal of viscous cell concentrates.

Single use centrifuge system for highly concentrated and/or turbid feeds

Vaporization of volatile materials while avoiding combustion and denaturation of such material provide an alternative to combustion as means of volatilizing bioactive and flavor compounds to make such compounds available for inhalation without generating toxic or carcinogenic substances that are by-products of combustion and pyrolysis.

Growing community of inventors

Magnolia, MA, United States of America

T David Marro