Sage Science, Inc.

Mosaic Technologies Inc.

Washington University

Matrix Technologies Corporation

Boles, T. Christian

Abrams, Ezra S.

Sabin, Douglas Grosvenor

Barbera, Todd J.

Yun, Danny

T Christian Boles

Todd J Barbera

Douglas Grosvenor Sabin

Ezra Solomon Abrams

Christopher P Adams

Paul Sabin

Stephen Joseph Kron

Joshua Gomes

Charles Sidoti

Andrew R Muir

Philip W Hammond

Simranjit Singh

Danny Yun

Jeffrey D Milbrandt

Robi David Mitra

Coimbatore Nagaraja Sridhar

Paul C Sabin

An electrophoresis cassette may include sample well(s), gel column(s) containing a separation gel, and elution modules arranged adjacent the gel column(s). A sample may be provided to the electrophoresis cassette and high-molecular weight (HMW) DNA may be isolated from the sample. Single-copy DNA sequences may be cleaved on both sides of a repeat region of the DNA sequences to produce a cleaved sample, which then may be fractionated using gel electrophoresis. DNA fractions may be isolated from consecutive sections of the separation gel and subjected to PCR assays to detect single-copy sequences within the DNA fraction, said single-copy sequence containing repeat expansion sequences. The subjected DNA fractions may be electroeluted into the plurality of elution modules. A size of DNA fractions having the repeat expansion sequences may be determined. It is also determined if that size is above a normal repeat size range.

Systems and methods for detection of genetic structural variation using integrated electrophoretic DNA purification

A sample containing particles having high-molecular-weight (HMW) DNA is entrapped in a gel matrix, and the gel matrix is exposed to a lysis reagent configured to release the HMW DNA from the particles. The HMW DNA may be purified by subjecting the gel matrix to an electrophoretic field that removes the HMW DNA from the particles, lysis reagents, and/or other sample constituents, from the gel matrix such that the HMW DNA remains. The gel matrix may be subjected with DNA cleavase reagents configured to cleave at specific DNA sequences within the HMW DNA to liberate defined segments of the DNA as fragments of reduced size. The gel matrix may also be subjected to an electrophoretic field, which moves and separates the DNA fragments from uncleaved DNA of the HMW DNA, which remains substantially immobile. The electrophoretically separated DNA fragments may be isolated from the gel matrix.

Preparative electrophoretic method for targeted purification of genomic DNA fragments

Methods, systems and apparatus for automated extraction, purification, and processing of nucleic acids from biological samples are presented. In some embodiments, hydrogel supports are used to immobilize particulate biological input samples and extract nucleic acids during operations. The use of hydrogel facilitates automated sample processing on robotic liquid handling systems. Devices, methods, and systems are also provided for electrophoretic sample preparation.

Apparatuses, methods and systems for automated processing of nucleic acids and electrophoretic sample preparation

The disclosure provides cassettes, electrophoresis systems, methods for making the device, and methods of fractionating a sample using the cassettes and electrophoresis systems described herein.

Side-eluting molecular fractionator

The invention provides an electrophoresis cassette, methods for making the electrophoresis cassette, and method of fractionating analytes from a sample based upon electrophoretic mobility in a single application of the sample to an electrophoretic system.

Multichannel preparative electrophoresis system

Systems and methods for processing fluid samples are disclosed. Fluid sample processing is accomplished using a series of microfluidic bump arrays include an automated and integrated system for sorting particles from a biological sample, lysing those particles to expose total RNA or DNA, purifying the RNA or DNA, processing the RNA or DNA by chemical or enzymatic modification, to select RNA or DNA molecules by size, or to generate, optionally, a sequencing library. The sequencing library is suitable for use in next generation sequencing ('NGS').

Systems and methods for processing fluids

Disclosed is a polymerizable complex containing a covalently attached nucleic acid molecule which, under appropriate conditions, is capable of copolymerization with a second polymerizable ethylene-containing monomer unit to form a polymerized layer. The polymerized layer containing attached nucleic acid is useful in a variety of contexts including, for example, hybridization assays. The polymerized layer containing the covalently attached nucleic acid molecule can be formed into a variety of shapes, or attached to a formed material through appropriate chemistry.

Nucleic acid-containing polymerizable complex

A multi-stage bridge amplification method which uses a recovered single-stranded amplification single-stranded nucleic acid molecule to initiate second and subsequent stages of bridge amplification is described.

Solid phase methods for amplifying multiple nucleic acids

Methods for determining the presence of a target polynucleotide sequence using chemical hybridization in sequential probe and displacement complex formation with potential for signal gain prior to detection are disclosed.

Multiple sequential polynucleotide displacement reactions for signal amplification and processing

Devices and methods for detecting the presence, or absence of the presence of at least one target molecule employing a receptacle housing a reaction chamber comprised of at least one compartment containing suitable reagents for the detection of the target molecule are disclosed.

Devices and methods for detecting target molecules in biological samples

Methods are described for separation and analysis, and a kit for separation and analysis, of single nucleotide polymorphisms, or mutations, in target nucleic acid using denaturing gradient electrophoresis through supporting media containing one or more immobilized nucleic acid capture ligand. The method is especially useful for analyzing genetic haplotypes in samples with multiple linked polymorphic sites.

Growing community of inventors

Bedford, MA, United States of America

T Christian Boles