Chemometec A/s

Søren Kjærulff

Martin Glensbjerg

Mette Elena Skindersø

Helle Frobøse Sørensen

Frans Ejner Ravn Hansen

Johan Holm

Frans Ejner Ravn

Rune Troelsgaard Pedersen

Helle Frobøse SØRENSEN

The current invention relates to the task of masking, i.e. determination of boundaries in an image of biological particles and/or elements or parts of biological particles in image cytometry. Methods for masking of a biological particle or element or region of a biological particle in a sample are provided which include staining a first element or region of the biological particle with a first fluorescent dye, staining a second element or region of the biological particle with a second fluorescent dye, recording an image of the fluorescent light signal emitted from the sample, and determining boundaries of the biological particle or element or region of the biological particle based on the fluorescent light signal in the image.

Masking of images of biological particles

The present invention relates to methods and systems for image cytometry analysis, typically at low optical magnification, where analysis is based on detection of biological particles using UV bright field and optionally one or more sources of excitation light.

Image forming cytometer

The present invention provides simple, rapid methods and procedures for analyzing cells, hereunder quantitative and qualitative assessment of cells, such as viability. The present invention relates to the use of various optionally substituted reporter compounds particularly detectable upon their reaction with thiol-containing species present in higher concentrations in intact (e.g., living) cells than in non-intact (e.g., dead, stressed and apoptotic) cells. The present invention also relates to the use of various optionally substituted reporter compounds particularly detectable upon their reaction with species present in intact and/or non-intact cells. Moreover, the present invention relates to the use of measuring techniques and/or instruments coupled with the use of various optionally substituted reporter compounds. The invention further relates to compositions used in methods for analyzing cells, such as a composition comprising N-(7-dimethylamino-4-methyl-3-coumarinyl)-maleimide (DACM).

Method and kit for assessing viable cells

According to an embodiment of the invention, an apparatus to detect fluorescence from a sample is disclosed. The apparatus comprises a sample plane onto which the sample is arranged, an excitation light unit including at least a light source to illuminate the sample, and a detection unit comprising at least a detector having at least 100,000 active detection elements to detect a fluorescence signal from the sample.

Growing community of inventors

Hillerød, Denmark

Søren Kjærulff