Life Technologies As

Life Technologies Corporation

Sintef

Synne Larsen

Geir Fonnum

Steven Menchen

Alfred Lui

Lars Kilaas

Ruth Schmid

Heidi Johnsen

Muhammed Talha Gokmen

Wilhelm Glomm

Nils Pontus Lundberg

Wolfgang Hinz

Prasanna Krishnan Thwar

Astrid Evenroed Molteberg

Lily Lu

Guobing Xiang

Pontus Lundberg

Lene Husaboe

Elisabeth Breivold

M Talha Gokmen

Talha M Gokmen

Per Nils Pontus Lundberg

Steven M Menchen

Muhammed Talha GÖKMEN

This invention relates to monodisperse cross-linked hydrogel polymer particles comprising a polymer formed from (a) a hydrophilic vinylic monomer; and (b) a crosslinker comprising at least two vinyl groups. The invention also relates to monodisperse seed particles with a Z-average diameter of from 100 nm to 1500 nm that comprise a plurality of non-crosslinked oligomers of poly N,N-dimethylacrylamide. Also provided are methods of forming the monodisperse cross-linked hydrogel polymer particles and monodisperse seed particles.

Inverse Ugelstad particles

Inverse ugelstad particles

A polymer substrate, such as a polymer coating or a polymer hydrogel network, includes carboxyl moieties that can be used as conjugation sites to which receptor or analyte molecules can be attached. In an example, the polymer substrate includes a polyacrylamide polymer network having alkanoic acid moieties or derivatives thereof, which can react with carboxyl activating compounds to provide an activated alkanoate moieties on the polyacrylamide network. Amine-terminated nucleic acids can react with the activated alkanoate moieties to capture the nucleic acid to the polymer network through an alkylamide moiety.

Conjugation of carboxyl functional hydrophilic beads

A polymer substrate, such as a polymer particle, is formed from a carboxyl functional monomer. In an example, the carboxyl functional monomer has a protection group in place of the OH of the carboxyl group. Once the monomer is polymerized, such a protection group can be removed, providing a polymer network with carboxyl functional sites. Such sites can be used to attach other functionality to the polymer substrate.

Polymer substrates formed from carboxy functional acrylamide

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Oslo, Norway

Synne Larsen