Centre National De La Recherche Scientifique

Commissariat a L'energie Atomique

Commissariat a L'energie Atomique Et Aux Energies Alternatives

Cis Bio International

Sylvie Sauvaigo

Jean-Paul Issartel

Didier Molko

Florence Marciacq

Jean-François Mouret

Robert Teoule

Thierry Livache

Herv&eacute; Bazin

Brigitte Fouque

Jean-François Millau

A method for quantitative assessment of base excision repair (BER) and nucleotide excision repair (NER) DNA capacities of one or more cellular extracts using supercoiled plasmid DNA by characterizing lesions on the plasmids relative to a control.

Method for the quantitative assessment of global and specific DNA repair capacities of at least one biological medium, and the applications therefor

A method for fixing a supercoiled DNA consists in deposing a supercoiled DNA sample on the surface of a porous polymer film, in fixing said supercoiled DNA therein by a passive diffusion, in obtaining a support by the inventive method and in using said support for analyzing the DNA distribution.

Method for fixing a supercoiled DNA and the use for analyzing the DNA repair

The invention relates to the use of morpholino-nucleosides of formula: in which Rrepresents a nucleic base and Rrepresents a group corresponding to one of the following formulae:&#x2014;(CH)&#x2014;NH&#x2014;(CH)&#x2014;SH&#x2014;(CH)&#x2014;COOH &#x2014;(CH)&#x2014;OH&#x2014;(CH)&#x2014;NH&#x2014;R&#x2014;(CH)&#x2014;SR&#x2014;(CH)&#x2014;CO&#x2014;R&#x2014;(CH)&#x2014;ORin which n is an interger ranging from 1 to 12 and Ris a group derived from a label, from a protein, from an enzyme, from a fatty acid or from a peptide, as chain terminators in a process of DNA or RNA sequencing by the Sanger method, or for the labelling of DNA or RNA fragments.

Process for manufacturing morpholino-nucleotides, and use thereof for the analysis of and labelling of nucleic acid sequences

The invention relates to the use of morpholino-nucleosides of formula: in which Rrepresents a nucleic base and Rrepresents a group corresponding to one of the following formulae:&#x2014;(CH)&#x2014;NH&#x2014;(CH)&#x2014;SH&#x2014;(CH)&#x2014;COOH &#x2014;(CH)&#x2014;OH&#x2014;(CH)&#x2014;NH&#x2014;R&#x2014;(CH)&#x2014;SR&#x2014;(CH)&#x2014;CO&#x2014;R&#x2014;(CH)&#x2014;OR

The invention relates to the use of morpholino-nucleosides of formula:in which Rrepresents a nucleic base and Rrepresents a group corresponding to one of the following formulae:

The invention concerns nucleic acid detection using a probe consisting of: a binding domain A, consisting of a single strand polynucleotide; and intermediate domain B, consisting of at least a stop synthon; and a display domain C, consisting of a double strand polynucleotide, wherein is incorporated at least a marked nucleotide constituting detection means. The use of said probes amplifies the hybridisation signal with nucleic acid target sequences.

System for detecting nucleic acid hybridization, preparation method and application thereof

A method for detecting specific nucleic acid sequences (a single sequence and/or a mixture of nucleic acid sequences) which are present in a biological sample, comprising at least one enzymatic amplification. Detection is carried out, after placing the biological samples in a solution in order to extract the nucleic acid, by enrichment with target sequences by putting the biological samples into contact with at least one pair of appropriate triggers, followed by at least one appropriate dilution of the obtained enrichment amplifying solution, and by putting a fraction of this solution into contact with at least one pair of triggers, followed by the detection of the obtained double-strand target nucleic acid copies. Applications in the diagnosis of genetic, infectious and tumoral diseases, in checking biological samples and in cell typing etc.

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Herbeys, France

Sylvie Sauvaigo