Institut National De La Recherche Agronomique (Inra)

Other

Institut National De Recherche Pour L'agriculture, L'alimentation Et L'environnement

Danstar Ferment Ag

Institut National De La Recherche Agronomique

Sylvie Dequin

Anne Ortiz-Julien

Pierre Barre

Virginie Ansanay

Jessica Noble

Maria Rosario Fernández Gallegos

Maryam Ehsani

Valentin Tilloy

Josep A Biosca

Axelle Cadiere

Maria Rosario FÉRNANDEZ Gallegos

The present disclosure concerns a process for obtaining a variant yeast strain capable of producing less ethanol in an alcoholic fermentation process than its corresponding ancestral strain. The variant yeast strain is obtained by culturing the ancestral strain in the presence of increasing concentrations of a salt capable of causing an hyperosmotic stress to the ancestral yeast strain. The present disclosure also concerns variant yeast strain obtained from this process (for example the variant yeast strain deposited at Institut Pasteur, on Jan. 9, 2014, under accession number CNCM I-4832, the variant yeast strain deposited at Institut Pasteur, on Oct. 18, 2012 under accession number CNCM I-4684, the variant yeast strain deposited at Institut Pasteur, on Oct. 18, 2012 under accession number CNCM I-4685 and/or the variant yeast strain deposited at Institut Pasteur on Jan. 28, 2015 under accession number CNCM I-4952) as well as processes using the variant yeast strain (wine fermentation for example).

Method for obtaining low ethanol-producing yeast strains, yeast strains obtained therefrom and their use

The invention relates to yeasts expressing the gene BDH1 coding for Bdh1p, characterized in that they catalyze, in an alcoholic fermentation medium, the reduction of acetoin into 2,3-butanediol by a rate at least twice higher than that of the initial stem. The invention can particularly be used for the fermentation of fruit juice and for producing 2,3-butanediol.

Means for reducing acetoin buildup in alcoholic fermentation media

Yeast strains expressing the lactic lacticodeshydrogenase gene and

Cloning and expression of the gene of the malolactic enzyme of

Growing community of inventors

Montpellier, France

Sylvie Dequin