Commissariat a L'energie Atomique

Commissariat &#xc0; L'&#xc9;nergie Atomique Et Aux &#xc9;nergies Alternatives

Sylvie Chevillard

Nicolas Ugolin

Jerome LeBeau

Nadine Coulon

Catherine Ory

Julien Le Meur

Denis Menut

Romain Grall

Olivier Limousin

Pascal Wodling

Hugues Girard

Jean-Charles Arnault

Jozo Delic

Caroline Falck

Emilie Lefevre

Tristan Petit

Emilie Bosc

Jean-Marc Joseph Desaulniers

Alexadre Coutant

Diana Renaud

Daniel Maier

Mory Doukoure

Olivier Alibert

Alexandre Coutant

Jérôme Lebeau

An apparatus for performing nanoparticle-assisted external beam radiotherapy includes an X-ray spectrometer having an optical axis, an X-ray filter, and a mobile holding structure for holding the X-ray filter and X-ray spectrometer in a first and second relative position, and for switching from the first to second relative position while simultaneously positioning a patient body part in a target region including a target point. An X-ray beam emitted from the X-ray source crosses the X-ray filter before reaching the target point. The propagation of the X-ray beam forms an angle different from 0&#xb0; and 180&#xb0; with the optical axis of the X-ray spectrometer. The second relative position is obtained by inverting the relative positions of the X-ray filter and X-ray spectrometer relative to the patient body part. A method of determining an X-ray dose delivered at a region of a patient body using the apparatus is provided.

Apparatus for performing nanoparticle-assisted external beam radiotherapy and method carried out using said apparatus

The present invention relates to the use of nanodiamonds as drugs generating free radicals, in particular for treating tumors. The invention is based on generating free radicals on the surface of the nanodiamonds when they are exposed to radiation, for example ionizing radiation. In order to increase the effectiveness of the nanodiamonds, the nanodiamonds can be complexed with a radiosensitizing agent, such as a chemical molecule or an interfering RNA targeting a repairing gene.

Use of nanodiamonds for generating free radicals for therapeutic purposes under radiation

A process, for the quantitative analysis by optical emission spectroscopy of a plasma induced by a laser beam of at least one target included in a biochip, involves the use of an adjuvant allowing the formation of a dry matrix capable of being ablated simultaneously with the at least one target, the dry matrix being configured to improve the analytical properties of plasma, the adjuvant having an emission spectrum whose lines have wavelengths distinct from the wavelengths of the spectral lines used for the quantitative analysis of said at least one target.

Method and device for the quantitative LIBS measurement of bio-molecular targets on a biochip

The present invention relates to a device and method for the high speed quantitative measurement of biomolecular targets on the surface or in the body of a planar medium for biological analysis. The method, according to the invention, includes the following, steps: a) at least two laser beams (F&#x2033;) are focused and overlaid on each measuring point of said medium by the simultaneous intersection of these beams to extract a contained hot plasma (P), including a measured chemical element present in the targets and another chemical element exogenous to the targets and present in a known quantity on this medium; b) luminous emission rays for each plasma, corresponding to the quantified element and exogenous element, are detected and analyzed for each measuring point while measuring the brightness of these rays; then c) the concentration in each measuring point of the quantified element is determined through prior calibration of the rays of the qualified element to determine a correlation between the brightness of the rays, specific to said element, and the concentrations of the latter in mixtures of the quantified element and exogenous element in known proportions.

Method and device for high speed quantitative measurement of biomolecular targets on or in biological analysis medium

The present invention relates in particular to a method for the quantitative measurement of biomolecular targets that have been deposited on a biochip () of the type with a matrix of probes hybridized by the targets, the matrix comprising a multitude of measurement points () each comprising a plurality of probes, characterized in that it comprises the following steps: a) at least one laser beam () is focused onto each measurement point, in order to extract therefrom a hot confined plasma comprising a chemical element to be quantified that is present in the targets and optionally in the probes; b) the light emission lines from the plasma are detected and analysed for each measurement point, by measuring the respective intensities of these lines; and then c) the concentration in each measurement point of the element or of a group incorporating it within the targets is determined via a prior calibration of the lines establishing a correlation between the intensities of the lines specific to the element to be quantified and given concentrations of this element.

Method for the quantitative measurement of biomolecular targets deposited on a biochip, and device for implementing it

The invention relates to a method of analysing molecular targets contained in a complex mixture, comprising the following steps consisting in: a) bringing the mixture of molecular targets to be analysed into contact with an array of different types of primary probes, whereby each type of primary probe forming the array can bind specifically to a type of target selected from among the molecular targets, under conditions that enable specific binding between the molecular targets and the primary probes; b) optionally eliminating the primary probes that are not bound specifically to a molecular target; c) separating the molecular targets and the primary probes which are bound specifically in a probe/target complex, such as to recover the array of primary probes representing a fingerprint of the molecular targets to be analysed; and d) quantitatively analysing the primary probes eluted in step c.

Method and device for separating molecular targets in a complex mixture

The invention relates to a device for separating and/analyzing several molecular targets dissolved in a complex mixture which is characterized in that it comprises

Device for separating and/or analyzing several molecular targets dissolved in a complex mixture

The invention relates to an image analysis process for measuring the signal on biochips organized into one or several blocks each comprising a large number of spots each composed of at least one probe, the said process being characterized in that it comprises the following steps:

Image analysis process for measuring the signal on biochips

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Le Kremlin-Bicetre, France

Sylvie Chevillard