Dsm IP Assets B.v.

Sylvia Hopper

Luppo Edens

Herman Jan Pel

Dieter Maier

Fabio Spreafico

Christian Wagner

Ulrike Folkers

Andreas Fritz

Kaj Albermann

Oliver Heinrich

Wolfram Kemmner

Alexander Stock

Richard Albang

Sabine Klugbauer

Wilk Von Gustedt

Pamela Tan

Josephine Stiebler

Albertus Van Dijk

Philipp Krubasik

Erik Kimpel

Four new genes, and the enzymes that they encode, are disclosed, which are present inand which are involved in at least five steps in the biosynthetic pathway of vitamin B. The four enzymes are: A: cobyrinic acid a, c-diamide synthase; B: a bifunctional enzyme, which is a cobinamide kinase and a cobinamide phosphate guanyl transferase; C: a cobalamin 5-phosphate synthase; and D: an adenosyl transferase. Genes encoding the four enzymes can be placed inshuttle vectors and used to transformhosts, in order to improve the production of vitamin Bor a precursor thereof on an industrial scale during fermentation.

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The invention relates to newly identified gene sequences that encode novel proteases obtainable from. The invention features the full length gene sequence of the novel genes, their cDNA sequences as well as the full-length functional protein and fragments thereof. The invention also relates to methods of using these enzymes in industrial processes and methods of diagnosing fungal infections. Also included in the invention are cells transformed with DNA according to the invention and cells wherein a protease according to the invention is genetically modified to enhance or reduce its activity and/or level of expression.

Genes encoding novel proteolytic enzymes

Growing community of inventors

Munich, Germany

Sylvia Hopper