Biomerieux, Inc.

Centre National De La Recherche Scientifique

UniversitÉde Strasbourg

Université De Caen Normandie

Centre International De Recherche Aux Frontieres De La Chimie

Sylvain Ursuegui

Ali Laayoun

Alain Laurent

Frédéric Fabis

Alain Wagner

Andrew David Griffiths

Michael Ryckelynck

Ketty Pernod

Thomas Cailly

Michaël Ryckelynck

The present invention concerns reagents for the reversible protection of biological molecules. It relates in particular to compounds derived from azaisatoic anhydride and their uses for the protection of biological molecules, particularly enzymes, in order to block their activity. The invention also relates to the biological molecules protected in this manner and to the methods for making use of these reagents.

Reagents for reversibly protecting biological molecules

The present invention relates to a method for capturing a molecular target present in the aqueous phase of a water-in-oil emulsion, said method being based on the use of a binding system comprising (a) a surfactant bearing a functional moiety on its hydrophilic head group and (b) a chemoprobe that acts as a molecular staple between the functionalized surfactant and the molecular target and comprises at least two distinct domains namely (i) at least one capture moiety which is able to specifically bind the molecular target and (ii) at least one binding domain which is able to interact with the functional group of the surfactant through covalent or non-covalent interactions, directly or through a binding intermediary.

Intra-droplet surface engineering to capture a molecular target

The present invention relates to a method of functionalization of at least one ribonucleic acid (RNA) molecule, contained in a liquid sample, which includes the following steps: a) providing at least: one binding molecule consisting of an aza-isatoic anhydride or a derivative thereof, one group of interest, and one linkage joining the binding molecule to the group of interest, b) reacting the anhydride function of the binding molecule with at least one hydroxyl group carried: in position 2' of the ribose of one of the RNA nucleotides, and/or in position(s) 2&#x2032; and/or 3&#x2032; of the ribose of the nucleotide at the terminal 3&#x2032; end of the RNA, and obtaining an aza-anthranilate that joins, by means of the linkage, the RNA to the group of interest.

Methods of functionalization and reagents used in such methods using an aza-isatoic anhydride or a derivative thereof, biological molecules thus treated and kits

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Strasbourg, France

Sylvain Ursuegui