Biomerieux, Inc.

Biomerieux Sa

Centre National De La Recherche Scientifique

Université´de Rouen

Biomerieux D.p.i.

Sylvain Orenga

John Perry

Arthur James

Stephen Stanforth

Vindhya Salwatura

Marie Cellier

Olivier Fabrega

Denis Robichon

Celine Roger-Dalbert

Gilles Zambardi

Céline Roger-Dalbert

Daniel Monget

David Mosticone

Antoine Vimont

Lyle Armstrong

Marie-Pierre Bourguignon

Diane Halimi

Vanessa Chanteperdrix

John Mills

Nathalie Bal

Anthony Romieu

Rosaleen Joy Anderson

Pierre-Yves Renard

Yuping Guo

Michel Peyret

Veronique Sauvonnet

Kerry Asir

Marine Casse

Christine Cotte

Alexandre F Bedernjak

Andréa Re

Sandra Gilbert

Linda Varadi

Mark Gray

Valérie Chalansonnet

Arnaud Chevalier

Rosaleen Anderson

Benoit Roubinet

Keng Tiong Ng

Laszlo Kondacs

Audrey Perry

Clair Preece

Martine Dhedin

Vindhya Lakshika Salwatura

John D Perry

Linda VÁRADI

Nathalie Bal Brossard

CÉLINE Roger-Dalbert

Véronique Sauvonnet

A new compound exhibiting particularly advantageous antibacterial properties, and having the structural formula: L-pyroglutamyl-L-1-aminoethyltetrazole (or N-(1-(1H-tetrazol-5-yl)ethyl)-5-oxopyrrolidine-2-carboxamide). Also, the use of this compound in microbiological culture methods and media intended for the detection, identification, enrichment, counting and/or isolation of target microorganisms that may be encountered in samples taken for clinical diagnosis or microbiological health control purposes.

Antibacterial compound and uses of same

A method for distinguishing among a first group of microorganisms belonging to a first taxon of Gram negative bacteria, the first group of bacteria exhibiting a mechanism of resistance to a treatment; a second group of microorganisms belonging to a second taxon of Gram negative bacteria, the second taxon of bacteria being different than said first taxon, and exhibiting a mechanism of resistance to a treatment identical to the mechanism of the first group; and a third group of Gram negative bacteria that is not resistant to the treatment.

Medium for the specific detection of resistant microorganisms

A microorganism detection medium, said detection being based on showing the presence of a microbial enzyme activity chosen from esterase and/or osidase and/or peptidase and/or sulfatase and/or phosphatase activities of microorganisms, preferably said microbial enzyme activity being an esterase activity, said medium comprising:

Microorganism detection method comprising at least one alkyl(thio)glycoside

Use of at least one chromogenic and/or fluorogenic phosphatase substrate for the detection and/or enumeration of enterobacteria in a sample likely to contain them, such as a food sample.

Use of at least one chromogenic and/or fluorogenic phosphatase substrate for the detection and/or enumeration of enterobacteria in a sample

A process for the enrichment and selective culture of mycobacteria contained in a biological sample, wherein all or part of the sample is inoculated in/on a culture medium including a nutritive component suitable for the development and growth of mycobacteria, wherein the culture medium includes, as selective agents, at least 9-chloro-9-(4'-diethylamino)phenyl-9,10-dihydro-10-phenylacridine hydrochloride (or C-390) and an agent capable of inhibitingbacteria. The invention also relates to a culture medium suitable for implementing this process for the enrichment and selective culture of mycobacteria.

Enrichment and selective culture of mycobacteria

Micro-organism detection medium comprising at least one alkyl(thio)glycoside

An antimicrobial compound, as well as the salts, derivatives and analogs thereof, said compound being represented by the general formula (I): wherein Rrepresents a peptide part P1 or a peptide part P2.

Antimicrobial compounds

The invention relates to fluorescent/fluorogenic probes of formula (I&#x2033;) or (II&#x2033;): wherein Z is chosen between:  It also relates to the use of these fluorescent/fluorogenic probes, for the detection of an enzyme activity, notably in order to identify/discriminate microorganisms in function of their ability to express particular enzyme activities.

Fluorogenic/fluorescent probes derivative from sulfoxanthene, and use thereof

The present invention relates to the field of microbiological testing of food. It relates to a kit comprising a reaction medium containing at least one inhibitor of Gram-negative bacteria and a fluorescent substrate specific for PC-PLC. It further relates to a diagnostic kit for identifying bacteria of thegroup comprising a container of a selective or nonselective reaction medium with a pH between 6.8 and 8.0, the medium comprising at least one inhibitor of Gram-negative bacteria and a fluorescent phosphatidylcholine phospholipase C (PC-PLC) substrate.

[object Object]

The invention relates to the use of a compound having formula (I) as an enzymatic substrate for the detection of a nitroreductase activity, wherein: W, W, Wand Ware independently H, Br, Cl, F, I, alkyl, alkoxy, thiomethyl, perfluoroalkyl, nitro, cyano, carboxyl (including the esters or amides thereof) or any combination of same; n=0, 1 or 2; X is NR, CZZ, S or O, R being H, alkyl, aralkyl, aryl, alkanoic or alkylsulphonic, Zand Zbeing an alkyl; Y is N or NR, R being alkyl, aralkyl, aryl, alkanoic or alkylsulphonic; Z, Z, Zand Zare independently H, Br, Cl, F, I, alkyl, aryl, alkoxy, perfluoroalkyl, nitro, cyano, carboxyl, sulphonyl, including the sulphonyl or carboxyl amides or esters thereof, and the salts of same. 

Nitroreductase enzymatic substrates

The present invention relates to a method for identifying bacteria of thegroup, comprising the following steps: (a) providing a sample that may contain bacteria of thegroup, a reaction medium comprising at least one fluorescent phosphatidylcholine phospholipase C (PC-PLC) substrate and an inhibitor of Gram-negative bacteria; (b) inoculating the reaction medium with the sample; (c) incubating the inoculated reaction medium; and (d) identifying the bacteria of thegroup by detecting the PC-PLC substrate hydrolysis reaction, in which the pH of the reaction medium and the time necessary for detecting the PC-PLC substrate hydrolysis reaction are adapted such that said hydrolysis reaction by bacteria of thegroup is detected before hydrolysis of the PC-PLC substrate by any Gram-positive bacteria other than those belonging to thegroup, that may be present in the sample.

A reaction medium for detecting and/or identifyingbacteria, comprising a combination of two enzymatic substrates for alpha-glucosidase.

A reaction medium for the culture and/or detection and/or identification of bacteria of thegenus includes at least one siliceous compound, where the siliceous compound is a nonpolar silica. A method for detecting and/or identifying bacteria of thegenus, includes bringing a sample that may contain bacteria of thegenus into contact with a reaction medium comprising at least one siliceous compound, incubating, and detecting the presence of bacteria of thegenus.

The invention relates to the use of a compound having formula (I) as an enzymatic substrate for the detection of a nitroreductase activity, wherein: W, W, Wand Ware independently H, Br, Cl, F, I, alkyl, alkoxy, thiomethyl, perfluoroalkyl, nitro, cyano, carboxyl (including the esters or amides thereof) or any combination of same; n=0, 1 or 2; U is N or NR and V is CZN or NR or V is N or NR and U is CZ, R being H, alkyl, aralkyl, aryl, alkanoic or alkylsulphonic; and Z, Z, Z, Z, Zand Zare independently H, Br, Cl, F, I, alkyl, aryl, alkoxy, perfluoroalkyl, nitro, cyano, carboxyl, sulphonyl, including the sulphonyl or carboxyl amides or esters thereof, and the salts of same.

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Neuville sur Ain, France

Sylvain Orenga