Universitéde Montreal

Sylvain Meloche

Anne Marinier

Pierre Thibault

Benoit Pelletier

Frédéric Galisson

Louiza Mahrouche

Eric Bonneil

Mounira Chelbi-Alix

Frederic Galisson

An assay and system compatible with high throughput screening (HTS) that is capable of identifying inhibitors, such as small-molecule inhibitors, of the degradation of the Cdk inhibitor p21, are described. The assay is based on the use of fusion protein comprising (i) a p2 polypeptide; and (i) a reporter protein linked to the C-terminal of said p21 polypeptide, wherein the fusion protein has a half-life that is similar to that of the p21 polypeptide. Inhibitors identified by this assay may be useful to inhibit the proliferation of tumor cells, and thus for the treatment of cancers.

Assay for inhibitors of CIP/KIP protein degradation

Disclosed herein are substantially pure nucleic acids encoding mutated SUMO isoforms, polypeptides, vectors, cells and methods of their use to identify and quantify protein SUMOylation in mammalian cells. Also disclosed is a dual affinity method for detecting a mutated SUMOylated protein substrate fragment.

Mutated SUMO isoforms and uses thereof

Growing community of inventors

Montreal, Canada

Sylvain Meloche