The United States of America As Represented by the Secretary of

The United States of America As Represented by the Department of Health

Clinical Technologies, Inc.

Daiichi Pharmaceutical Co., Ltd.

The Government of the United States of America, As Represented by the

Syed Zaki Salahuddin

Robert C Gallo

Flossie Wong-Staal

Carl W Saxinger

Dharam V Ablashi

Steven F Josephs

Nickolas Chelyapov

Shuji Nakamura

Noriko G Tanaka

Yasuaki Osada

Shinsaku Sakurada

A recombinant human immunodeficiency virus (rHIV) and recombinant mammalian cell-line (packaging systems) and a method for treating infection of cells by human immuneodeficiency virus HIV. rHIV, such as rHIV-1, comprises of a gene construction which includes a foreign gene. The expression of this gene is activated in human cells in the presence of wild-type HIV. This gene product can cause cell death in the presence of an appropriate drug, e.g. Acyclovir. This gene product is typically a viral thymidine kinase. rHIV is so constructed that it is unable to replicate by itself due to the absence of a regulatory gene that is necessary for its replication, such as tat or rev or both. The recombinant mammalian cell-line packaging system comprises in its genome a recombinant gene construction which typically includes a functional regulatory gene from HIV which is missing from rHIV, such as the tat or rev genes or both. The method for treating infection of cells by HIV comprises administering a composition comprising rHIV, which is produced in vitro from a recombinant mammalian cell-line, and treating with a nucleoside analog, such as Acyclovir or Gancyclovir.

Recombinant HIV and modified packaging cells and method for using

A new human B lymphotropic virus, also designated human herpesvirus-6, has been isolated. DNA, molecular clones, antigenic viral proteins and antibodies having specificity to the new virus have been prepared. Various utilities of the new virus and products derived therefrom have been described.

Antibodies against human herpesvirus-6(HHV-6) and method of use

Human herpesvirus-6 (HHV-6) isolation and products

Recombinant HIV and modified packaging cells and method for treating

Human herpesvirus-6(HHV-6)Isolution and Products

The present invention is directed to a method for arresting or inhibiting the growth of cells in Kaposi's Sarcoma lesions and a method for arresting or inhibiting the growth of the Kaposi's Sarcoma lesions, said methods comprising contacting the cells in the lesions with an effective amount of SP-PG, a naturally occurring sulfated polysaccharide-peptidoglycan produced by a specific species of the bacterium Arthrobacter, AT-25. The invention is also directed to blocking or inhibiting the activity of cellular vascular permeability factor(s), which comprises contacting vascular cells with an effective amount of SP-PG. In one embodiment, there is provided a method for blocking or inhibiting increased vascular permeability (and resulting edema) in diseases and disorders in which the increased vascular permeability contributes to the pathology, for example, in Kaposi's Sarcoma, tumorigenesis, inflammation, diabetic retinopathy, etc. Increased effectiveness is obtained when SP-PG is combined with cortisone or a cortisone derivative, such as hydrocortisone or tetrahydrocortisone.

Method for treating Kaposi's sarcoma and blocking or inhibiting vascular

The present invention relates to a growth factor found in HTLV-II conditioned media and to compositions containing same. The growth factor of the present invention supports the growth of Kaposi's sarcoma endothelial-like cells. The factor has a molecular weight of 30K to 35K in monomeric form and a molecular weight of about 70K in dimeric form.

Kaposi's sarcoma endothelial cells and growth factor

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Pasadena, CA, United States of America

Syed Zaki Salahuddin