Takara Bio Usa, Inc.

Sotax Corporation

Wafergen, Inc.

Automated Semiconductor, Inc.

Syed A Husain

Jude Menlo Dunne

Bradley L Griswold

Colin B Kennedy

Dale Von Behren

Maithreyan Srinivasan

Wesley B Dong

GoPaul Kotturi

Philip Lin

Michael Slater

Glenn Hein

Patricio A Espinoza Vallejos

Rick Bernal

Robert Moti

Chun-Wah Lin

Patricio Espinoza

Hermann Hubschle

Jude Dunne

Saad Mukhtar

Enrique Bernal

Provided are methods, devices, assemblies, and systems for dispensing into the wells of a multi-well device and imaging such wells from multiple Z-planes. Multi-Z imaging of the present methods and systems may allow for the detection of wells of a multi-well device that contain a desired number of cells. Also provided are methods, devices, assemblies, and systems for processing cell-containing wells of a multi-well device identified through the use of multi-Z imaging.

Multi-Z imaging and dispensing with multi-well devices

The present invention provides methods, systems, assemblies, and articles for extracting restrained liquid (e.g., surface tension-restrained liquid) from open wells in a chip, where the restrained liquid does not flow out of the wells due to gravity when the wells are held upside down. For example, the present invention provides extraction fixtures that may be attached to, and/or held adjacent to, a chip such that any restrained liquid that is forced out of the open wells is collected by, or flows through, the extraction fixtures. Also for example, the present invention provides assemblies composed of a extraction fixture attached to, and/or held adjacent to, a chip, and methods of subjecting such assemblies to a force such that at least a portion of the restrained liquid in the open wells is forced out and collected by, or flows through, the extraction fixture.

Extraction of restrained liquid from wells

The present invention provides methods, systems, assemblies, and articles for capturing single cells with a polymer capture film. In certain embodiments, the polymer capture films comprise a plurality of individual channels with top and bottom openings, where the channels are dimensioned such that a single cell is: i) is captured inside the channel, partially or substantially occluding the channel, when negative pressure is provided to the bottom opening; or ii) is captured by the top opening, but does not enter the channel, when negative pressure is provided to the bottom opening. In some embodiments, the channels of the polymer capture film align with the wells of a multi-well chip such that the cell, or the contents of the single cell, may be transferred to a corresponding well.

Single cell capture with polymer capture films

The present invention provides methods, systems, assemblies, and articles for capturing single cells with a capture chip. In certain embodiments, the capture chip comprises a substrate comprising a plurality of cell-sized dimples or wells that each allow a single cell to be captured from a cell suspension. In some embodiments, the dimples or wells of the capture chip align with the holes or wells of a multi-well through-hole chip, and/or a multi-well chip, such that the cell, or the contents of the single cell, may be transferred to a corresponding well of the multi-well chip. In particular embodiments, the bottom of each dimple or well of the capture chip has a positive electrical charge sufficient to attract cells from a cell suspension flowing over the dimples or wells.

Single cell capture with capture chips

The invention provides a system and method for dissolution testing. The system includes multiple dissolution vessels and a dose carrier positioned above the dissolution vessels. The dose carrier holds multiple removable carousels that receive individual doses for dissolution tested. Carousels that receive tablets or sinkers typically have a first configuration, while carousels that receive baskets typically have a second configuration. The two different configurations of carousels are interchangeable on the same dose ring. The system further includes a drive head positioned above the dose carrier, the drive head having a basket arbor and a mixing paddle removably and interchangeably attached. A pipettor integral with the system transfers sample aliquots having volumes in the range of 50 &#x3bc;l to 1 ml from the dissolution vessels to wells of an external receptacle.

Integrated dissolution processing and sample transfer system

A selective plating apparatus having a multipitch centerline feed block for delivering a uniform blade of plating solution to a mask and which provides passageways for the drainage of used electrolyte solution. The delivery of a uniform blade of plating solution to the mask allows the mask to be designed such that it controls both the area of sample to be plated and the pitch of the sample.

Universal selective plating head

Growing community of inventors

Fremont, CA, United States of America

Syed A Husain