Agency for Science, Technology and Research

National University of Singapore

Singapore Health Services Pte Ltd

Tan Tock Seng Hospital Pte Ltd

Swaine Chen

Roger Wilmer Beuerman

Chandra Shekhar Verma

Tiang Hwee Donald Tan

Lakshminarayanan Rajamani

Timothy Barkham

Mercy Halleluyah Periayah

Jianguo Li

Thet Tun Aung

Majid Eshaghi

Jun Jie Koh

The present invention relates to a compound comprising a hydrophobic moiety, a linker and N-containing moiety. The present invention also relates to the method of synthesizing the compound and the use of the compound as an antibiotic or an adjuvant for an antibiotic.

Molecular design of new antibiotics and antibiotic adjuvants against MCR strains

We describe the rational structure-based design of monomeric and dimeric forms of a nanobody-enhanced GFP (termed vsfGFP) that demonstrates &#x2dc;1.3-fold higher brightness than sfGFP in a monomeric form and &#x2dc;2.5-fold higher brightness in a dimeric form. These new vsfGFP variants demonstrate high stability and brightness in both bacterial and eukaryotic cells and are thus ideal for in vivo imaging applications. The combination of higher brightness, facile folding, stable expression, and tunable dimerization makes them ideal partners in essentially all in vitro applications already described for fluorescent proteins, including antibody fusion-based molecular probes, for which the higher brightness and tunable dimerization provide distinct advantages. Furthermore, the vsfGFP variants retain folding properties of sfGFP that enable bright fluorescence in oxidizing environments such as the bacterial periplasm. In particular, periplasmic expression enables the general construction of functional, fluorescent single domain antibody fusions, markedly enhancing the breadth and utility and reducing the cost of these molecular probes. Generalization of the vGFP strategy should provide similar improvements to other fluorescent proteins and may be extendable to higher order (and thus higher brightness) complexes.

Nanobody-flourescent protein fusion

Growing community of inventors

Singapore, Singapore

Swaine Chen