Bio-rad Laboratories, Inc.

Hercules Corporation

Svilen Tzonev

Claudia Litterst

Dianna Maar

John Frederick Regan

Yann Jouvenot

Geoff McDermott

Ryan T Koehler

Benjamin J Hindson

Serge Saxonov

Jeremy Agresti

Michael Ybarra Lucero

George Karlin-Neumann

Adam McCoy

Frank Hsiung

Adam M Mccoy

Provided herein are methods, compositions, systems, and kits for recombination assays, many of which involve amplification reactions such as PCR or droplet digital PCR.

Genetic assays

The present invention provides methods of detecting a target molecule in a sample comprising incubating the sample with two or more detectably labeled probes, partitioning the sample into multiple partitions, and detecting the presence of the two or more probes in the same partition.

Affinity-based partition assay for detection of target molecules

Digital assay system, including methods, apparatus, and compositions, for performing target assays in partitions each containing a generic reporter and a specific reporter for target amplification.

Multiplexed digital assay with specific and generic reporters

Methods compositions and kits are provided for performing a chromatin or chromosome conformation capture assay in partitions.

Chromosome conformation capture in partitions

Digital assay system, including methods and apparatus, for calculating a level of one or more targets. In an exemplary method, data for amplification of a plurality of targets including a first target may be collected from partitions. A level of the first target may be calculated from only a subset of the data that excludes partitions according to target content.

Multiplexed digital assay with data exclusion for calculation of target levels

System, including methods, apparatus, and compositions, for performing a multiplexed digital assay of at least three targets. In an exemplary method, partitions may be formed each including a portion of a same sample. The sample may include a first target that is a reference sequence, a second target that is a variant sequence from a gene of interest, and a third target that is a normal sequence from the gene of interest. The target may be amplified in the partitions and amplification data may be collected. A level of the second target may be determined. A copy number of the third target may be determined from a level of the first target and a level of the third target.

Multiplexed digital assay for variant and normal forms of a gene of interest

Methods of characterizing a nucleic acid library by digital assay.

Growing community of inventors

Pleasanton, CA, United States of America

Svilen Tzonev