Ajinomoto Co., Ltd.

Svetlana Aleksandrovna Fomina

Maria Grigorievna Lunts

Mikhail Markovich Gusyatiner

Tatyana Viktorovna Leonova

Vera Georgievna Doroshenko

Sergei Vladimirovich Mashko

Aleksandra Yurievna Skorokhodova

Joanna Yosifovna Katashkina

Lirina Valerievna Ivanovskaya

A method is provided for obtaining an L-amino acid or nucleic acid-producing bacterium belonging to the genuswith an optimized level of expression of the gene which influences the distribution of carbon flow, such as the sucAB genes, comprising introducing into the chromosome of the bacterium a set of in vitro constructed DNA fragments which contain regulatory elements for gene expression instead of the native elements of the regulatory region of the gene, and selecting the colonies with increased L-amino acid productivity. Also, a method is provided for producing an L-amino acid, such as L-glutamic acid, L-proline, L-arginine, L-glutamine, L-leucine, using the bacterium with an optimized level of expression of the sucAB gene.

Method for producing an L-amino acid using a bacterium with an optimized level of gene expression

L-Glutamic acid, L-proline or L-arginine is produced by culturing a bacterium belonging to the genus, which is L-isoleucine auxotrophic and has ability to produce L-glutamic acid, L-proline or L-arginine, in a medium containing L-isoleucine, to produce and accumulate L-glutamic acid, L-proline or L-arginine in a culture, and collecting L-glutamic acid, L-proline or L-arginine from the culture.

Bacterium having ability to produce L-glutamic acid, L-proline or L-arginine and method for producing L-glutamic acid, L-proline or L-arginine

L-Glutamic acid, L-proline or L-arginine is produced by culturing a bacterium belonging to the genus Escherichia, which is L-isoleucine auxotrophic and has ability to produce L-glutamic acid, L-proline or L-arginine, in a medium containing L-isoleucine, to produce and accumulate L-glutamic acid, L-proline or L-arginine in a culture, and collecting L-glutamic acid, L-proline or L-arginine from the culture.

Growing community of inventors

Moscow, Russia

Svetlana Aleksandrovna Fomina