The United States of America, As Represented by the Secretary, Department of Health and Human Services

Washington University

Suxiang Tong

Larry J Anderson

Thomas G Ksiazek

Anthony Sanchez

Pierre E Rollin

Raymond Campagnoli

M Steven Oberste

Cara Carthel Burns

William Allan Nix

Paul A Rota

Dean D Erdman

James A Comer

Mark A Pallansch

William J Bellini

Cynthia Goldsmith

Stephan S Monroe

Sherif R Zaki

Qi Chen

Shannon L Emery

Charles D Humphrey

Joseph P Icenogle

Teresa C T Peret

David Huabin Wang

Herbert Virgin, Iv

Yan Li

Guoyan Zhao

Wun-Ju Shieh

Michael D Bowen

Byron T Cook

Jeanette Guarner

Richard F Meyer

Stacy Finkbeiner

Christopher D Paddock

Jan Vinje

Disclosed herein are methods of detecting influenza virus in a sample from a subject. In some embodiments, the disclosed methods include contacting a sample with at least one primer 10-40 nucleotides in length wherein the at least one primer is capable of hybridizing to an influenza virus polymerase basic protein 1 (PB1) nucleic acid at least 70% identical to the nucleic acid sequence set forth as any one of SEQ ID NOs: 1-3, amplifying the PB1 nucleic acid or a portion thereof to produce an amplified PB1 nucleic acid, and detecting the amplified PB1 nucleic acid, wherein presence of the amplified PB1 nucleic acid indicates presence of influenza virus in the sample from the subject. In some examples, the primers comprise or consist of the nucleic acid sequence set forth as one of SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 9, or SEQ ID NO: 10.

Methods of detecting influenza virus

Provided herein is a novel human astrovirus, its nucleic acid sequence, as well as methods to detect and diagnose the presence of the astrovirus.

Identification of astrovirus VA1 associated with gastroenteritis in humans

Disclosed herein is a newly isolated human coronavirus (SARS-CoV), the causative agent of severe acute respiratory syndrome (SARS). Also provided are the nucleic acid sequence of the SARS-CoV genome and the amino acid sequences of the SARS-CoV open reading frames, as well as methods of using these molecules to detect a SARS-CoV and detect infections therewith. Immune stimulatory compositions are also provided, along with methods of their use.

Coronavirus isolated from humans

Growing community of inventors

Duluth, GA, United States of America

Suxiang Tong